Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-21) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM5720385	M	M	NA	Agree	0.00	6.51	0.00	42.16	12.22	72.31	NONE	POLYA	0.865359	0.081474	0.038538	0.014629	94.51	2.81	2.50	88	LP2 (2026-09-30)	PASS	NonPolyA_perM 88 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5720386	M	M	NA	Agree	0.02	6.54	0.00	35.09	14.00	67.76	NONE	POLYA	0.877289	0.076742	0.038971	0.006998	92.98	2.09	4.77	85	LP2 (2026-09-30)	PASS	NonPolyA_perM 85 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5720387	M	M	NA	Agree	0.01	7.22	0.00	41.04	13.20	69.02	NONE	POLYA	0.857221	0.08646	0.03928	0.01704	92.71	3.05	4.06	120	LP2 (2026-09-30)	PASS	NonPolyA_perM 120 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5720388	M	M	NA	Agree	0.00	4.57	0.00	36.34	9.80	71.86	NONE	POLYA	0.894693	0.051636	0.034658	0.019012	93.43	3.35	3.07	63	LP2 (2026-09-30)	PASS	NonPolyA_perM 63 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5720389	M	M	NA	Agree	0.01	7.17	0.00	38.19	9.87	76.10	NONE	POLYA	0.885305	0.067552	0.037656	0.009487	93.78	2.32	3.73	73	LP2 (2026-09-30)	PASS	NonPolyA_perM 73 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5720390	M	M	NA	Agree	0.00	6.65	0.00	35.16	10.54	77.55	NONE	POLYA	0.89319	0.059863	0.040332	0.006615	94.83	2.06	2.94	73	LP2 (2026-09-30)	PASS	NonPolyA_perM 73 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5720391	M	M	NA	Agree	0.04	7.56	0.00	45.99	15.42	62.17	NONE	POLYA	0.839659	0.099976	0.045187	0.015177	94.12	2.83	2.85	123	LP2 (2026-09-30)	PASS	NonPolyA_perM 123 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5720392	M	M	NA	Agree	0.06	5.70	0.00	43.15	11.66	69.11	NONE	POLYA	0.891782	0.05571	0.037569	0.014939	93.05	2.75	4.05	55	LP2 (2026-09-30)	PASS	NonPolyA_perM 55 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5720393	M	M	NA	Agree	0.01	6.20	0.00	32.09	13.78	61.38	NONE	POLYA	0.873069	0.063926	0.037974	0.02503	64.30	2.78	32.79	59	LP2 (2026-09-30)	PASS	NonPolyA_perM 59 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5720394	M	M	NA	Agree	0.02	6.38	0.00	36.41	13.56	63.11	NONE	POLYA	0.893052	0.065012	0.03732	0.004616	87.94	1.65	10.27	55	LP2 (2026-09-30)	PASS	NonPolyA_perM 55 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5720395	M	M	NA	Agree	0.02	6.19	0.00	36.19	12.38	62.39	NONE	POLYA	0.873794	0.076718	0.037699	0.011789	86.07	2.28	11.49	67	LP2 (2026-09-30)	PASS	NonPolyA_perM 67 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5720396	M	M	NA	Agree	0.02	6.94	0.00	39.97	12.81	64.67	NONE	POLYA	0.878623	0.06954	0.038393	0.013444	86.12	2.39	11.34	77	LP2 (2026-09-30)	PASS	NonPolyA_perM 77 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5720397	M	M	NA	Agree	0.02	7.77	0.00	34.97	13.68	58.93	NONE	POLYA	0.879854	0.071445	0.034829	0.013872	85.07	2.46	12.31	69	LP2 (2026-09-30)	PASS	NonPolyA_perM 69 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5720398	M	M	NA	Agree	0.02	5.76	0.00	34.99	12.05	68.64	NONE	POLYA	0.893536	0.056254	0.035592	0.014619	80.14	2.46	17.26	68	LP2 (2026-09-30)	PASS	NonPolyA_perM 68 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does