Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-21) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM5705303	F	F	NA	Agree	77.65	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.892416	0.056048	0.041892	0.009643	93.18	3.19	3.36	146	LP2 (2026-09-30)	PASS	NonPolyA_perM 146 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5705304	F	F	NA	Agree	69.71	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.885713	0.063719	0.040066	0.010501	93.05	3.17	3.57	134	LP2 (2026-09-30)	PASS	NonPolyA_perM 134 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5705305	F	F	NA	Agree	75.25	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.863667	0.071528	0.04261	0.022195	92.55	3.96	3.26	127	LP2 (2026-09-30)	PASS	NonPolyA_perM 127 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5705306	F	F	NA	Agree	50.91	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.879792	0.060513	0.043806	0.015889	91.78	3.66	4.34	137	LP2 (2026-09-30)	PASS	NonPolyA_perM 137 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5705307	F	F	NA	Agree	48.03	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.899365	0.045037	0.044931	0.010666	91.62	3.72	4.42	133	LP2 (2026-09-30)	PASS	NonPolyA_perM 133 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5705308	F	F	NA	Agree	45.10	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.896684	0.043039	0.0403	0.019977	91.02	4.46	4.23	124	LP2 (2026-09-30)	PASS	NonPolyA_perM 124 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does