Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-22) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM5590217	F	F	NA	Agree	99.64	0.00	0.00	0.00	0.00	0.00	NONE	AMBIGUOUS	0.669632	0.283133	0.039786	0.007449	90.36	3.12	6.26	445	LP2 (2026-09-30)	PASS	NonPolyA_perM 445 is between 350 and 700, outside the range of every calibration study; check the study methods
GSM5590218	F	F	NA	Agree	126.85	0.00	0.00	0.00	0.00	0.00	NONE	AMBIGUOUS	0.544552	0.36459	0.041257	0.049601	87.12	6.29	6.25	639	LP2 (2026-09-30)	PASS	NonPolyA_perM 639 is between 350 and 700, outside the range of every calibration study; check the study methods
GSM5590219	F	F	NA	Agree	105.62	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.716724	0.233733	0.042502	0.007041	89.92	3.79	5.98	307	LP2 (2026-09-30)	PASS	NonPolyA_perM 307 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5590220	F	F	NA	Agree	99.76	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.728277	0.219616	0.043976	0.008131	87.53	4.94	7.13	278	LP2 (2026-09-30)	PASS	NonPolyA_perM 278 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5590221	F	F	NA	Agree	99.79	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.762991	0.187326	0.044197	0.005485	88.41	3.66	7.59	227	LP2 (2026-09-30)	PASS	NonPolyA_perM 227 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5590222	F	F	NA	Agree	119.59	0.00	0.00	0.00	0.00	0.00	NONE	AMBIGUOUS	0.682313	0.264236	0.045514	0.007937	89.57	3.36	6.70	409	LP2 (2026-09-30)	PASS	NonPolyA_perM 409 is between 350 and 700, outside the range of every calibration study; check the study methods
GSM5590223	F	F	NA	Agree	141.00	0.00	0.00	0.00	0.00	0.00	NONE	AMBIGUOUS	0.647836	0.297891	0.044123	0.01015	89.35	3.18	7.10	434	LP2 (2026-09-30)	PASS	NonPolyA_perM 434 is between 350 and 700, outside the range of every calibration study; check the study methods
GSM5590224	F	F	NA	Agree	87.79	0.00	0.00	0.00	0.00	0.00	NONE	AMBIGUOUS	0.700614	0.252777	0.041024	0.005585	86.30	3.64	9.71	381	LP2 (2026-09-30)	PASS	NonPolyA_perM 381 is between 350 and 700, outside the range of every calibration study; check the study methods
GSM5590225	F	F	NA	Agree	102.83	0.00	0.00	0.00	0.00	0.00	NONE	AMBIGUOUS	0.690934	0.254271	0.043848	0.010947	88.78	3.64	7.18	355	LP2 (2026-09-30)	PASS	NonPolyA_perM 355 is between 350 and 700, outside the range of every calibration study; check the study methods
GSM5590226	F	F	NA	Agree	92.07	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.697366	0.2449	0.045326	0.012409	87.92	5.07	6.60	342	LP2 (2026-09-30)	PASS	NonPolyA_perM 342 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5590227	F	F	NA	Agree	61.22	0.00	0.00	0.00	0.00	0.00	NONE	AMBIGUOUS	0.682011	0.266466	0.040621	0.010902	85.28	5.72	8.55	432	LP2 (2026-09-30)	PASS	NonPolyA_perM 432 is between 350 and 700, outside the range of every calibration study; check the study methods
GSM5590228	F	F	NA	Agree	96.48	0.00	0.00	0.00	0.00	0.00	NONE	AMBIGUOUS	0.696918	0.252763	0.040857	0.009462	87.53	4.36	7.72	369	LP2 (2026-09-30)	PASS	NonPolyA_perM 369 is between 350 and 700, outside the range of every calibration study; check the study methods
GSM5590229	F	F	NA	Agree	117.35	0.00	0.00	0.00	0.00	0.00	NONE	AMBIGUOUS	0.654213	0.29101	0.043646	0.011131	89.09	3.68	6.85	413	LP2 (2026-09-30)	PASS	NonPolyA_perM 413 is between 350 and 700, outside the range of every calibration study; check the study methods
GSM5590230	F	F	NA	Agree	141.68	0.00	0.00	0.05	0.02	0.00	NONE	AMBIGUOUS	0.656918	0.28989	0.044076	0.009116	89.91	3.68	6.03	438	LP2 (2026-09-30)	PASS	NonPolyA_perM 438 is between 350 and 700, outside the range of every calibration study; check the study methods
GSM5590231	F	F	NA	Agree	162.22	0.00	0.00	0.00	0.00	0.00	NONE	AMBIGUOUS	0.664998	0.281911	0.047942	0.005148	91.69	2.97	5.02	394	LP2 (2026-09-30)	PASS	NonPolyA_perM 394 is between 350 and 700, outside the range of every calibration study; check the study methods