Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-21) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM5572644	M	M	NA	Agree	0.00	2.35	0.00	11.76	4.76	49.60	NONE	POLYA	0.943187	0.023022	0.029029	0.004761	93.61	2.54	3.65	40	LP2 (2026-09-30)	PASS	NonPolyA_perM 40 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5572645	M	M	NA	Agree	0.00	3.39	0.00	12.65	5.28	49.48	NONE	POLYA	0.938426	0.023854	0.031858	0.005862	93.16	2.90	3.71	22	LP2 (2026-09-30)	PASS	NonPolyA_perM 22 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5572646	M	M	NA	Agree	0.00	3.14	0.00	12.85	6.09	53.65	NONE	POLYA	0.939939	0.026035	0.029103	0.004924	93.86	2.56	3.40	37	LP2 (2026-09-30)	PASS	NonPolyA_perM 37 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5572647	M	M	NA	Agree	0.03	3.65	0.00	15.99	6.39	57.53	NONE	POLYA	0.936767	0.027614	0.030897	0.004722	93.83	2.67	3.31	31	LP2 (2026-09-30)	PASS	NonPolyA_perM 31 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5572648	M	M	NA	Agree	0.01	6.34	0.00	17.83	8.46	75.43	NONE	POLYA	0.930654	0.033419	0.031406	0.004521	92.98	2.54	4.28	62	LP2 (2026-09-30)	PASS	NonPolyA_perM 62 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5572649	M	M	NA	Agree	0.00	4.88	0.00	16.84	7.72	67.29	NONE	POLYA	0.93178	0.033292	0.029754	0.005174	94.06	2.40	3.35	50	LP2 (2026-09-30)	PASS	NonPolyA_perM 50 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5572650	M	M	NA	Agree	0.00	4.41	0.00	9.93	7.76	76.42	NONE	POLYA	0.935309	0.024903	0.033868	0.005921	93.57	2.78	3.43	48	LP2 (2026-09-30)	PASS	NonPolyA_perM 48 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5572651	M	M	NA	Agree	0.01	5.24	0.00	15.84	9.09	74.16	NONE	POLYA	0.924323	0.036153	0.031291	0.008233	93.65	2.85	3.27	61	LP2 (2026-09-30)	PASS	NonPolyA_perM 61 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5572652	M	M	NA	Agree	0.00	3.32	0.00	13.06	5.09	60.58	NONE	POLYA	0.936215	0.028227	0.030395	0.005162	93.71	2.72	3.34	48	LP2 (2026-09-30)	PASS	NonPolyA_perM 48 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5572653	M	M	NA	Agree	0.01	4.12	0.00	15.19	7.90	58.63	NONE	POLYA	0.9324	0.033046	0.029792	0.004762	93.97	2.41	3.41	60	LP2 (2026-09-30)	PASS	NonPolyA_perM 60 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5572654	M	M	NA	Agree	0.00	3.75	0.00	10.71	6.51	55.84	NONE	POLYA	0.940597	0.024786	0.030887	0.00373	93.88	2.43	3.49	55	LP2 (2026-09-30)	PASS	NonPolyA_perM 55 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5572655	M	M	NA	Agree	0.00	3.35	0.00	10.64	6.47	60.36	NONE	POLYA	0.940151	0.025593	0.030736	0.00352	93.79	2.42	3.57	58	LP2 (2026-09-30)	PASS	NonPolyA_perM 58 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5572656	M	M	NA	Agree	0.03	6.01	0.00	16.45	8.53	77.19	NONE	POLYA	0.93831	0.027299	0.030579	0.003812	92.56	2.68	4.53	50	LP2 (2026-09-30)	PASS	NonPolyA_perM 50 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5572657	M	M	NA	Agree	0.01	4.95	0.00	16.00	10.28	71.88	NONE	POLYA	0.921638	0.042465	0.032265	0.003632	93.69	2.57	3.51	72	LP2 (2026-09-30)	PASS	NonPolyA_perM 72 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5572658	M	M	NA	Agree	0.02	4.62	0.00	16.05	8.65	68.86	NONE	POLYA	0.934028	0.030112	0.030998	0.004862	92.55	2.68	4.57	56	LP2 (2026-09-30)	PASS	NonPolyA_perM 56 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5572659	M	M	NA	Agree	0.00	4.97	0.00	15.53	7.76	69.43	NONE	POLYA	0.934276	0.029854	0.031489	0.00438	93.43	2.55	3.80	43	LP2 (2026-09-30)	PASS	NonPolyA_perM 43 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does