Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-22) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM5501302	M	M	NA	Agree	0.00	3.50	0.00	12.54	7.50	64.98	NONE	POLYA	0.916445	0.03597	0.03852	0.009065	92.84	3.35	3.61	77	LP2 (2026-09-30)	PASS	NonPolyA_perM 77 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5501303	M	M	NA	Agree	0.00	4.79	0.00	19.58	9.87	83.37	NONE	POLYA	0.914767	0.037168	0.040125	0.007941	93.60	3.06	3.13	55	LP2 (2026-09-30)	PASS	NonPolyA_perM 55 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5501304	M	M	NA	Agree	0.00	5.84	0.00	18.11	11.29	90.23	NONE	POLYA	0.921437	0.034771	0.037833	0.00596	93.77	2.74	3.26	58	LP2 (2026-09-30)	PASS	NonPolyA_perM 58 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5501305	M	M	NA	Agree	0.00	8.98	0.00	22.09	16.01	84.96	NONE	POLYA	0.912879	0.043974	0.039355	0.003792	94.99	2.30	2.48	65	LP2 (2026-09-30)	PASS	NonPolyA_perM 65 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5501306	M	M	NA	Agree	0.01	7.02	0.00	19.04	11.64	100.92	NONE	POLYA	0.913463	0.044891	0.036984	0.004662	94.57	2.62	2.60	92	LP2 (2026-09-30)	PASS	NonPolyA_perM 92 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5501307	M	M	NA	Agree	0.01	6.06	0.00	22.55	11.72	78.24	NONE	POLYA	0.932486	0.02967	0.034706	0.003139	94.65	2.19	2.97	54	LP2 (2026-09-30)	PASS	NonPolyA_perM 54 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5501308	M	M	NA	Agree	0.00	4.46	0.00	18.51	9.32	54.60	NONE	POLYA	0.909512	0.053814	0.034358	0.002315	95.04	1.98	2.74	63	LP2 (2026-09-30)	PASS	NonPolyA_perM 63 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5501309	M	M	NA	Agree	0.00	2.60	0.00	12.82	8.67	51.86	NONE	POLYA	0.920929	0.043474	0.029916	0.005681	94.16	2.50	3.10	52	LP2 (2026-09-30)	PASS	NonPolyA_perM 52 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5501310	M	M	NA	Agree	0.02	3.56	0.00	17.39	6.28	59.15	NONE	POLYA	0.906962	0.051981	0.038003	0.003054	94.03	2.88	2.74	64	LP2 (2026-09-30)	PASS	NonPolyA_perM 64 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5501311	M	M	NA	Agree	0.03	6.91	0.00	21.07	14.21	89.27	NONE	POLYA	0.907902	0.047072	0.039338	0.005689	94.10	2.73	2.93	81	LP2 (2026-09-30)	PASS	NonPolyA_perM 81 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5501312	M	M	NA	Agree	0.00	2.90	0.00	7.81	6.73	54.14	NONE	POLYA	0.907389	0.042812	0.043159	0.00664	93.59	2.92	3.26	78	LP2 (2026-09-30)	PASS	NonPolyA_perM 78 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5501313	M	M	NA	Agree	0.00	3.97	0.00	9.65	10.37	63.19	NONE	POLYA	0.900553	0.054335	0.040881	0.00423	94.65	2.55	2.54	102	LP2 (2026-09-30)	PASS	NonPolyA_perM 102 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5501314	M	M	NA	Agree	0.02	7.95	0.00	21.24	16.04	92.34	NONE	POLYA	0.911062	0.044027	0.039373	0.005537	94.47	2.42	2.87	74	LP2 (2026-09-30)	PASS	NonPolyA_perM 74 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5501315	M	M	NA	Agree	0.00	10.23	0.00	26.71	16.02	86.95	NONE	POLYA	0.892172	0.060951	0.042142	0.004735	93.68	2.46	3.62	80	LP2 (2026-09-30)	PASS	NonPolyA_perM 80 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5501316	M	M	NA	Agree	0.02	8.36	0.00	24.09	14.53	94.37	NONE	POLYA	0.912356	0.043793	0.039073	0.004779	93.70	2.54	3.55	64	LP2 (2026-09-30)	PASS	NonPolyA_perM 64 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does