Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-20) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM5217481	M	M	NA	Agree	0.01	14.10	0.00	63.76	25.17	104.92	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.823194	0.121153	0.055112	0.000541	97.36	1.89	0.51	119	LP2 (2026-09-30)	PASS	NonPolyA_perM 119 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5217482	M	M	NA	Agree	0.01	13.32	0.00	60.88	25.55	94.71	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.821544	0.123776	0.054188	0.000492	97.35	1.84	0.59	126	LP2 (2026-09-30)	PASS	NonPolyA_perM 126 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5217483	M	M	NA	Agree	0.00	13.04	0.00	68.41	25.25	94.84	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.825551	0.12007	0.05372	0.000659	97.23	1.77	0.78	108	LP2 (2026-09-30)	PASS	NonPolyA_perM 108 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5217484	M	M	NA	Agree	0.03	12.33	0.00	63.80	25.52	105.47	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.832597	0.112088	0.05464	0.000674	97.28	1.89	0.61	113	LP2 (2026-09-30)	PASS	NonPolyA_perM 113 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5217485	M	M	NA	Agree	0.01	12.32	0.00	54.40	26.09	98.64	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.827956	0.116505	0.054669	0.00087	97.38	1.89	0.51	125	LP2 (2026-09-30)	PASS	NonPolyA_perM 125 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5217486	M	M	NA	Agree	0.01	13.49	0.00	66.41	26.58	103.23	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.825143	0.12014	0.053999	0.000718	97.29	1.90	0.57	121	LP2 (2026-09-30)	PASS	NonPolyA_perM 121 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5217487	M	M	NA	Agree	0.03	12.91	0.00	56.21	20.82	100.48	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.838518	0.108517	0.052358	0.000606	97.31	1.89	0.58	103	LP2 (2026-09-30)	PASS	NonPolyA_perM 103 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5217488	M	M	NA	Agree	0.01	12.64	0.00	61.18	24.67	97.20	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.829582	0.116643	0.053256	0.000519	97.41	1.85	0.51	124	LP2 (2026-09-30)	PASS	NonPolyA_perM 124 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5217489	M	M	NA	Agree	0.01	13.90	0.00	58.14	22.00	92.60	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.821468	0.121803	0.054864	0.001865	97.26	1.99	0.53	127	LP2 (2026-09-30)	PASS	NonPolyA_perM 127 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5217490	M	M	NA	Agree	0.01	12.83	0.00	58.97	20.28	100.01	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.829396	0.117436	0.052641	0.000527	97.30	1.92	0.54	98	LP2 (2026-09-30)	PASS	NonPolyA_perM 98 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5217491	M	M	NA	Agree	0.02	10.92	0.00	58.70	26.61	103.48	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.83132	0.114451	0.053581	0.000649	97.40	1.86	0.52	118	LP2 (2026-09-30)	PASS	NonPolyA_perM 118 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5217492	M	M	NA	Agree	0.01	11.82	0.00	60.66	23.55	106.39	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.825169	0.120982	0.053178	0.000671	97.43	1.85	0.49	133	LP2 (2026-09-30)	PASS	NonPolyA_perM 133 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5217493	M	M	NA	Agree	0.00	13.49	0.00	70.34	21.20	98.64	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.823741	0.122975	0.052352	0.000932	97.40	1.90	0.48	151	LP2 (2026-09-30)	PASS	NonPolyA_perM 151 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5217494	M	M	NA	Agree	0.01	11.92	0.00	62.94	24.29	99.60	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.831483	0.114572	0.053044	0.000901	97.37	1.90	0.50	107	LP2 (2026-09-30)	PASS	NonPolyA_perM 107 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5217495	M	M	NA	Agree	0.01	12.07	0.00	61.42	25.00	97.60	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.838358	0.10879	0.052344	0.000509	97.40	1.86	0.51	117	LP2 (2026-09-30)	PASS	NonPolyA_perM 117 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5217496	M	M	NA	Agree	0.00	12.23	0.00	59.50	24.13	98.16	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.825409	0.119845	0.054144	0.000602	97.34	1.89	0.55	119	LP2 (2026-09-30)	PASS	NonPolyA_perM 119 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5217497	M	M	NA	Agree	2.41	11.96	0.00	68.08	21.97	97.23	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.823137	0.121784	0.054533	0.000545	97.32	1.93	0.51	110	LP2 (2026-09-30)	PASS	NonPolyA_perM 110 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5217498	M	M	NA	Agree	0.01	12.15	0.00	64.08	22.56	97.35	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.834311	0.112761	0.052409	0.000519	97.32	1.88	0.58	114	LP2 (2026-09-30)	PASS	NonPolyA_perM 114 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does