Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-22) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM5216024	M	M	NA	Agree	0.05	4.34	0.00	32.30	7.25	39.73	NONE	POLYA	0.961285	0.023824	0.013514	0.001378	96.57	1.58	1.69	18	LP2 (2026-09-30)	PASS	NonPolyA_perM 18 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5216025	M	M	NA	Agree	0.04	4.28	0.00	24.15	4.89	33.20	NONE	POLYA	0.960461	0.024985	0.013228	0.001325	96.93	1.45	1.44	31	LP2 (2026-09-30)	PASS	NonPolyA_perM 31 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5216026	M	M	NA	Agree	0.06	4.07	0.00	30.13	6.60	42.45	NONE	POLYA	0.95648	0.02852	0.013881	0.001119	96.32	1.41	2.09	32	LP2 (2026-09-30)	PASS	NonPolyA_perM 32 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5216027	M	M	NA	Agree	0.03	2.58	0.00	27.65	5.49	24.74	NONE	POLYA	0.95927	0.025505	0.01373	0.001495	97.42	1.18	1.28	58	LP2 (2026-09-30)	PASS	NonPolyA_perM 58 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5216028	M	M	NA	Agree	0.03	3.45	0.00	31.69	5.03	30.89	NONE	POLYA	0.96289	0.022524	0.013032	0.001554	96.75	1.22	1.93	33	LP2 (2026-09-30)	PASS	NonPolyA_perM 33 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5216029	M	M	NA	Agree	0.08	4.94	0.00	42.14	5.85	39.74	NONE	POLYA	0.951264	0.031455	0.01566	0.001621	96.57	1.31	2.00	63	LP2 (2026-09-30)	PASS	NonPolyA_perM 63 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does