Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-22) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM5207248	F	F	NA	Agree	185.16	0.00	0.00	0.21	0.00	0.16	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.852063	0.107117	0.022544	0.018276	81.08	3.83	14.76	66	LP2 (2026-09-30)	PASS	NonPolyA_perM 66 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5207249	F	F	NA	Agree	90.58	0.05	0.00	0.03	0.00	0.00	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.889884	0.08381	0.022382	0.003924	85.30	2.48	11.97	60	LP2 (2026-09-30)	PASS	NonPolyA_perM 60 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5207250	F	F	NA	Agree	261.91	0.03	0.00	0.39	0.00	0.00	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.834222	0.138076	0.026008	0.001695	86.09	2.37	11.21	98	LP2 (2026-09-30)	PASS	NonPolyA_perM 98 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5207251	F	F	NA	Agree	171.24	0.00	0.00	0.00	0.04	0.00	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.880656	0.095414	0.022375	0.001555	86.08	2.21	11.35	78	LP2 (2026-09-30)	PASS	NonPolyA_perM 78 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5207252	F	F	NA	Agree	141.77	0.00	0.00	0.00	0.00	0.16	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.870555	0.105497	0.022762	0.001186	86.01	2.66	11.02	99	LP2 (2026-09-30)	PASS	NonPolyA_perM 99 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5207253	F	F	NA	Agree	97.85	0.00	0.00	0.00	0.03	0.00	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.896509	0.080419	0.022176	0.000896	87.61	2.67	9.43	74	LP2 (2026-09-30)	PASS	NonPolyA_perM 74 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5207254	F	F	NA	Agree	104.78	0.00	0.00	0.02	0.00	0.00	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.895777	0.08115	0.021683	0.00139	87.60	2.29	9.76	55	LP2 (2026-09-30)	PASS	NonPolyA_perM 55 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5207255	F	F	NA	Agree	241.04	0.04	0.00	0.60	0.63	1.51	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.839975	0.133138	0.025573	0.001314	86.55	2.24	10.83	86	LP2 (2026-09-30)	PASS	NonPolyA_perM 86 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5207256	F	F	NA	Agree	254.01	0.00	0.00	0.00	0.00	0.00	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.839942	0.130033	0.025778	0.004248	82.81	2.57	14.32	99	LP2 (2026-09-30)	PASS	NonPolyA_perM 99 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5207257	F	F	NA	Agree	180.92	0.06	0.00	0.07	0.00	0.00	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.872679	0.094838	0.022287	0.010196	85.97	2.94	10.85	54	LP2 (2026-09-30)	PASS	NonPolyA_perM 54 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5207258	F	F	NA	Agree	204.47	0.00	0.00	0.09	0.00	0.00	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.849241	0.114006	0.023822	0.012931	86.23	3.78	9.57	67	LP2 (2026-09-30)	PASS	NonPolyA_perM 67 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5207259	F	F	NA	Agree	240.54	0.00	0.00	0.05	0.00	0.00	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.858756	0.117134	0.022706	0.001403	86.34	2.26	10.99	93	LP2 (2026-09-30)	PASS	NonPolyA_perM 93 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5207260	F	F	NA	Agree	182.44	0.00	0.00	0.04	0.00	0.00	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.876248	0.098955	0.02353	0.001267	88.80	2.43	8.44	49	LP2 (2026-09-30)	PASS	NonPolyA_perM 49 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5207261	F	F	NA	Agree	62.26	0.00	0.00	0.02	0.00	0.00	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.925903	0.051728	0.020319	0.00205	89.26	2.22	8.27	44	LP2 (2026-09-30)	PASS	NonPolyA_perM 44 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5207262	F	F	NA	Agree	27.67	0.00	0.00	0.00	0.00	0.00	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.937731	0.038604	0.020229	0.003436	87.93	2.41	9.39	32	LP2 (2026-09-30)	PASS	NonPolyA_perM 32 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5207263	F	F	NA	Agree	70.36	0.02	0.00	0.00	0.00	0.18	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.914247	0.061861	0.021058	0.002834	87.73	2.74	9.23	53	LP2 (2026-09-30)	PASS	NonPolyA_perM 53 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5207264	F	F	NA	Agree	194.75	0.00	0.00	0.00	0.03	0.00	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.879151	0.09713	0.023089	0.000631	91.92	2.04	5.80	59	LP2 (2026-09-30)	PASS	NonPolyA_perM 59 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5207265	F	F	NA	Agree	127.16	0.00	0.00	0.00	0.04	0.00	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.898562	0.07789	0.023007	0.00054	90.38	2.82	6.54	53	LP2 (2026-09-30)	PASS	NonPolyA_perM 53 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5207266	F	F	NA	Agree	451.75	0.00	0.00	0.00	0.00	0.24	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.899602	0.080115	0.019369	0.000913	92.62	1.40	5.70	74	LP2 (2026-09-30)	PASS	NonPolyA_perM 74 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5207267	F	F	NA	Agree	303.25	0.00	0.00	0.00	0.00	0.00	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.838384	0.134131	0.026307	0.001177	88.23	2.25	9.12	76	LP2 (2026-09-30)	PASS	NonPolyA_perM 76 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5207268	F	F	NA	Agree	239.78	0.00	0.00	0.00	0.00	0.00	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.843659	0.128155	0.027409	0.000776	91.09	2.56	5.93	128	LP2 (2026-09-30)	PASS	NonPolyA_perM 128 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5207269	F	F	NA	Agree	213.48	0.00	0.00	0.00	0.04	0.00	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.836572	0.136212	0.026546	0.00067	91.15	2.50	5.89	128	LP2 (2026-09-30)	PASS	NonPolyA_perM 128 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5207270	F	F	NA	Agree	202.76	0.00	0.00	0.00	0.00	0.00	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.850169	0.123214	0.025612	0.001005	89.54	2.20	7.83	97	LP2 (2026-09-30)	PASS	NonPolyA_perM 97 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5207271	F	F	NA	Agree	125.25	0.00	0.00	0.04	0.00	0.07	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.891305	0.083878	0.024187	0.000631	89.64	2.91	7.14	80	LP2 (2026-09-30)	PASS	NonPolyA_perM 80 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does