Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-19) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM4947395	M	M	NA	Agree	0.04	8.64	0.00	49.87	32.00	53.55	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.846708	0.109905	0.04257	0.000817	93.76	3.29	2.36	126	LP2 (2026-09-30)	PASS	NonPolyA_perM 126 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4947396	M	M	NA	Agree	0.02	7.59	0.00	49.58	27.16	49.69	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.851954	0.106295	0.041321	0.00043	94.49	3.20	1.78	114	LP2 (2026-09-30)	PASS	NonPolyA_perM 114 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4947397	M	M	NA	Agree	0.03	8.36	0.00	46.79	27.02	58.06	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.879193	0.079856	0.039876	0.001076	94.02	3.46	2.08	93	LP2 (2026-09-30)	PASS	NonPolyA_perM 93 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4947398	M	M	NA	Agree	0.01	6.07	0.00	42.22	20.78	65.57	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.879995	0.079933	0.039197	0.000875	93.74	4.28	1.48	157	LP2 (2026-09-30)	PASS	NonPolyA_perM 157 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4947399	M	M	NA	Agree	0.02	7.16	0.00	42.20	22.06	59.46	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.869456	0.091456	0.038294	0.000795	93.64	4.27	1.60	127	LP2 (2026-09-30)	PASS	NonPolyA_perM 127 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4947400	M	M	NA	Agree	0.01	3.68	0.00	32.96	12.06	58.62	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.899416	0.058068	0.040585	0.001932	92.49	5.51	1.54	120	LP2 (2026-09-30)	PASS	NonPolyA_perM 120 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4947401	M	M	NA	Agree	0.01	6.78	0.00	40.10	26.70	53.00	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.868673	0.085055	0.038999	0.007274	90.00	4.25	5.27	115	LP2 (2026-09-30)	PASS	NonPolyA_perM 115 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4947402	M	M	NA	Agree	0.03	8.46	0.00	44.01	25.58	56.29	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.872516	0.084359	0.037568	0.005556	92.62	4.25	2.64	139	LP2 (2026-09-30)	PASS	NonPolyA_perM 139 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4947403	M	M	NA	Agree	0.00	4.46	0.00	25.88	18.26	51.03	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.876393	0.071204	0.042195	0.010208	89.95	5.87	3.55	136	LP2 (2026-09-30)	PASS	NonPolyA_perM 136 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4947404	M	M	NA	Agree	0.10	10.71	0.00	80.41	19.44	48.12	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.828498	0.12732	0.04143	0.002752	92.19	3.35	3.73	256	LP2 (2026-09-30)	PASS	NonPolyA_perM 256 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4947405	M	M	NA	Agree	0.10	11.95	0.00	92.84	18.00	52.23	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.820848	0.133564	0.042602	0.002986	91.61	3.23	4.36	281	LP2 (2026-09-30)	PASS	NonPolyA_perM 281 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4947406	M	M	NA	Agree	0.02	11.63	0.00	88.31	19.85	61.74	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.830424	0.126592	0.039236	0.003747	91.26	3.62	4.46	308	LP2 (2026-09-30)	PASS	NonPolyA_perM 308 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does