Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-22) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM4908785	M	M	NA	Agree	0.00	9.05	0.00	49.43	20.31	107.49	NONE	POLYA	0.902497	0.034862	0.046014	0.016627	88.40	3.00	8.46	50	LP2 (2026-09-30)	PASS	NonPolyA_perM 50 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4908786	M	M	NA	Agree	0.00	7.19	0.00	42.24	18.33	88.52	NONE	POLYA	0.897672	0.036033	0.045204	0.021091	87.32	3.46	9.10	53	LP2 (2026-09-30)	PASS	NonPolyA_perM 53 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4908787	M	M	NA	Agree	0.00	8.86	0.00	47.61	17.72	98.53	NONE	POLYA	0.894137	0.041038	0.044755	0.02007	87.66	3.33	8.87	58	LP2 (2026-09-30)	PASS	NonPolyA_perM 58 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4908788	M	M	NA	Agree	0.00	8.92	0.00	46.84	18.01	93.50	NONE	POLYA	0.89769	0.046909	0.046704	0.008697	88.82	2.25	8.79	73	LP2 (2026-09-30)	PASS	NonPolyA_perM 73 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4908789	M	M	NA	Agree	0.00	9.02	0.00	46.20	21.46	98.69	NONE	POLYA	0.903079	0.032543	0.046112	0.018266	88.40	3.17	8.30	55	LP2 (2026-09-30)	PASS	NonPolyA_perM 55 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4908790	M	M	NA	Agree	0.01	8.20	0.00	42.83	18.92	107.16	NONE	POLYA	0.900291	0.032585	0.046414	0.020709	88.45	3.43	7.98	37	LP2 (2026-09-30)	PASS	NonPolyA_perM 37 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4908791	M	M	NA	Agree	0.00	8.65	0.00	46.75	19.38	100.68	NONE	POLYA	0.898799	0.037611	0.04604	0.017551	88.65	3.06	8.15	50	LP2 (2026-09-30)	PASS	NonPolyA_perM 50 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4908792	M	M	NA	Agree	0.00	6.83	0.00	43.48	17.51	96.91	NONE	POLYA	0.88311	0.047072	0.044251	0.025568	86.55	3.84	9.48	73	LP2 (2026-09-30)	PASS	NonPolyA_perM 73 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4908793	M	M	NA	Agree	0.00	6.97	0.00	40.04	17.47	102.50	NONE	POLYA	0.895986	0.031875	0.043747	0.028392	87.16	4.17	8.54	47	LP2 (2026-09-30)	PASS	NonPolyA_perM 47 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does