Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-20) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM4903770	F	F	NA	Agree	148.37	0.01	0.00	0.03	0.01	0.00	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.814531	0.111504	0.047918	0.026048	91.52	4.21	4.01	112	LP2 (2026-09-30)	PASS	NonPolyA_perM 112 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4903771	F	F	NA	Agree	173.74	0.00	0.00	0.00	0.00	0.00	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.804498	0.118401	0.049535	0.027566	89.57	4.44	5.70	128	LP2 (2026-09-30)	PASS	NonPolyA_perM 128 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4903772	F	F	NA	Agree	154.26	0.01	0.00	0.06	0.01	0.11	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.697564	0.154125	0.045471	0.10284	82.48	11.27	5.94	182	LP2 (2026-09-30)	PASS	NonPolyA_perM 182 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4903773	F	F	NA	Agree	160.37	0.02	0.00	0.05	0.03	0.21	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.813807	0.11288	0.047308	0.026005	91.59	4.25	3.88	134	LP2 (2026-09-30)	PASS	NonPolyA_perM 134 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4903774	F	F	NA	Agree	148.57	0.03	0.00	0.04	0.04	0.11	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.819166	0.110804	0.047647	0.022384	91.78	3.95	3.98	124	LP2 (2026-09-30)	PASS	NonPolyA_perM 124 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4903775	F	F	NA	Agree	141.04	0.00	0.00	0.02	0.00	0.00	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.69286	0.165438	0.045589	0.096114	85.47	10.59	3.63	154	LP2 (2026-09-30)	PASS	NonPolyA_perM 154 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4903776	F	F	NA	Agree	142.70	0.00	0.00	0.02	0.03	0.00	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.819351	0.112071	0.047612	0.020965	92.30	3.76	3.66	112	LP2 (2026-09-30)	PASS	NonPolyA_perM 112 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4903777	F	F	NA	Agree	165.22	0.00	0.00	0.00	0.00	0.00	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.842388	0.107298	0.046239	0.004076	95.80	2.18	1.77	90	LP2 (2026-09-30)	PASS	NonPolyA_perM 90 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4903778	F	F	NA	Agree	173.14	0.00	0.00	0.00	0.00	0.00	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.836033	0.113702	0.046224	0.004042	95.66	2.22	1.85	107	LP2 (2026-09-30)	PASS	NonPolyA_perM 107 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4903779	F	F	NA	Agree	154.99	0.00	0.00	0.02	0.02	0.12	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.843027	0.106796	0.047008	0.003169	95.81	2.12	1.80	97	LP2 (2026-09-30)	PASS	NonPolyA_perM 97 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4903780	F	F	NA	Agree	141.64	0.00	0.00	0.07	0.06	0.00	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.841986	0.10765	0.047533	0.00283	95.25	2.09	2.39	93	LP2 (2026-09-30)	PASS	NonPolyA_perM 93 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4903781	F	F	NA	Agree	134.36	0.00	0.00	0.00	0.00	0.00	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.835872	0.114726	0.046138	0.003264	95.50	2.14	2.09	105	LP2 (2026-09-30)	PASS	NonPolyA_perM 105 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4903782	F	F	NA	Agree	117.51	0.00	0.00	0.05	0.03	0.00	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.837066	0.10866	0.050343	0.003932	92.54	2.32	4.85	99	LP2 (2026-09-30)	PASS	NonPolyA_perM 99 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4903783	F	F	NA	Agree	175.94	0.02	0.00	0.10	0.06	0.18	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.834814	0.114788	0.047144	0.003254	95.38	2.12	2.24	110	LP2 (2026-09-30)	PASS	NonPolyA_perM 110 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4903784	F	F	NA	Agree	157.12	0.00	0.00	0.03	0.04	0.04	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.841416	0.104952	0.046598	0.007034	94.87	2.48	2.33	90	LP2 (2026-09-30)	PASS	NonPolyA_perM 90 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4903785	F	F	NA	Agree	173.26	0.00	0.00	0.06	0.00	0.00	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.831759	0.114766	0.047252	0.006224	95.09	2.39	2.23	115	LP2 (2026-09-30)	PASS	NonPolyA_perM 115 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4903786	F	F	NA	Agree	202.14	0.00	0.00	0.03	0.00	0.00	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.829321	0.118429	0.049408	0.002842	94.19	2.05	3.44	114	LP2 (2026-09-30)	PASS	NonPolyA_perM 114 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4903787	F	F	NA	Agree	162.92	0.00	0.00	0.05	0.02	0.04	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.841556	0.108255	0.046707	0.003482	95.56	2.10	2.07	95	LP2 (2026-09-30)	PASS	NonPolyA_perM 95 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4903788	F	F	NA	Agree	165.15	0.00	0.00	0.07	0.00	0.14	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.836689	0.111228	0.046838	0.005245	95.03	2.29	2.34	100	LP2 (2026-09-30)	PASS	NonPolyA_perM 100 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4903789	F	F	NA	Agree	173.78	0.00	0.00	0.00	0.00	0.00	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.831064	0.116088	0.046678	0.006169	94.91	2.34	2.46	105	LP2 (2026-09-30)	PASS	NonPolyA_perM 105 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4903790	F	F	NA	Agree	153.27	0.00	0.00	0.00	0.00	0.00	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.835943	0.112635	0.047138	0.004283	95.55	2.16	2.02	113	LP2 (2026-09-30)	PASS	NonPolyA_perM 113 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4903791	F	F	NA	Agree	176.58	0.00	0.00	0.01	0.05	0.19	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.823295	0.12302	0.048694	0.004991	94.70	2.28	2.69	101	LP2 (2026-09-30)	PASS	NonPolyA_perM 101 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4903792	F	F	NA	Agree	166.12	0.03	0.00	0.05	0.04	0.08	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.833243	0.115066	0.047231	0.004461	95.92	2.16	1.64	99	LP2 (2026-09-30)	PASS	NonPolyA_perM 99 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4903793	F	F	NA	Agree	157.04	0.00	0.00	0.00	0.00	0.00	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.833441	0.115122	0.046286	0.005151	95.47	2.18	2.08	83	LP2 (2026-09-30)	PASS	NonPolyA_perM 83 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does