Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-21) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM4886641	M	M	NA	Agree	0.01	9.93	0.00	47.89	19.31	60.50	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.850898	0.092096	0.043767	0.013239	85.77	3.51	10.37	113	LP2 (2026-09-30)	PASS	NonPolyA_perM 113 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4886642	M	M	NA	Agree	0.10	11.14	0.00	50.91	21.98	77.09	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.856564	0.098612	0.04188	0.002945	86.49	2.43	10.78	128	LP2 (2026-09-30)	PASS	NonPolyA_perM 128 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4886643	M	M	NA	Agree	0.13	12.06	0.00	49.47	21.52	70.89	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.856722	0.09752	0.042398	0.003359	86.30	2.33	11.08	103	LP2 (2026-09-30)	PASS	NonPolyA_perM 103 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4886644	M	M	NA	Agree	0.14	9.10	0.00	45.19	21.49	57.75	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.845186	0.105822	0.045468	0.003525	86.00	2.54	11.15	91	LP2 (2026-09-30)	PASS	NonPolyA_perM 91 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4886645	M	M	NA	Agree	0.03	8.93	0.00	51.60	20.43	62.08	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.845642	0.10207	0.045464	0.006824	84.21	3.00	12.50	123	LP2 (2026-09-30)	PASS	NonPolyA_perM 123 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4886646	M	M	NA	Agree	0.06	9.40	0.00	40.20	23.53	60.86	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.848336	0.102875	0.046233	0.002556	86.12	2.71	10.81	104	LP2 (2026-09-30)	PASS	NonPolyA_perM 104 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4886647	M	M	NA	Agree	0.06	10.31	0.00	51.90	20.74	59.61	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.838644	0.110863	0.047332	0.003162	85.84	2.41	11.39	112	LP2 (2026-09-30)	PASS	NonPolyA_perM 112 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4886648	M	M	NA	Agree	0.05	9.08	0.00	46.09	19.46	56.99	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.858015	0.093552	0.045681	0.002753	85.82	2.44	11.46	84	LP2 (2026-09-30)	PASS	NonPolyA_perM 84 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4886649	M	M	NA	Agree	0.06	10.70	0.00	45.73	19.37	55.88	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.851142	0.10142	0.044664	0.002774	86.06	2.61	10.98	99	LP2 (2026-09-30)	PASS	NonPolyA_perM 99 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4886650	M	M	NA	Agree	0.04	11.70	0.00	57.20	23.23	62.49	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.834468	0.112858	0.046469	0.006205	86.02	2.68	11.00	90	LP2 (2026-09-30)	PASS	NonPolyA_perM 90 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4886651	M	M	NA	Agree	0.06	10.76	0.00	55.95	22.27	68.80	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.839075	0.110307	0.045885	0.004732	85.96	2.64	11.08	108	LP2 (2026-09-30)	PASS	NonPolyA_perM 108 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4886652	M	M	NA	Agree	0.07	9.78	0.00	39.84	22.45	63.23	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.834265	0.11369	0.047913	0.004132	85.42	2.62	11.59	128	LP2 (2026-09-30)	PASS	NonPolyA_perM 128 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does