Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-21) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM4874222	M	M	NA	Agree	0.01	2.91	0.00	25.00	12.75	48.78	NONE	POLYA	0.904977	0.051423	0.039047	0.004553	95.45	1.71	2.68	53	LP2 (2026-09-30)	PASS	NonPolyA_perM 53 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4874223	M	M	NA	Agree	0.01	3.20	0.00	28.24	9.73	52.96	NONE	POLYA	0.907048	0.041799	0.03782	0.013334	94.26	2.51	3.08	56	LP2 (2026-09-30)	PASS	NonPolyA_perM 56 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4874224	M	M	NA	Agree	0.01	3.60	0.00	33.61	6.74	42.37	NONE	POLYA	0.87521	0.072433	0.0452	0.007157	92.76	3.31	3.52	112	LP2 (2026-09-30)	PASS	NonPolyA_perM 112 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4874225	M	M	NA	Agree	0.01	4.42	0.00	26.74	9.14	52.12	NONE	POLYA	0.904347	0.056539	0.035523	0.003591	94.33	1.84	3.61	70	LP2 (2026-09-30)	PASS	NonPolyA_perM 70 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4874226	M	M	NA	Agree	0.01	3.63	0.00	24.18	9.77	54.02	NONE	POLYA	0.905165	0.052221	0.036278	0.006336	94.25	2.01	3.52	55	LP2 (2026-09-30)	PASS	NonPolyA_perM 55 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4874227	M	M	NA	Agree	0.00	3.62	0.00	26.93	12.80	60.43	NONE	POLYA	0.907543	0.048196	0.036393	0.007868	93.79	2.04	4.01	66	LP2 (2026-09-30)	PASS	NonPolyA_perM 66 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4874228	M	M	NA	Agree	0.00	3.91	0.00	30.76	10.06	58.09	NONE	POLYA	0.907688	0.04698	0.037775	0.007556	93.24	2.02	4.57	43	LP2 (2026-09-30)	PASS	NonPolyA_perM 43 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4874229	M	M	NA	Agree	0.01	3.19	0.00	29.17	10.31	49.22	NONE	POLYA	0.913199	0.042801	0.037313	0.006687	94.29	1.85	3.69	45	LP2 (2026-09-30)	PASS	NonPolyA_perM 45 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4874230	M	M	NA	Agree	0.02	4.03	0.00	27.89	10.19	51.66	NONE	POLYA	0.895644	0.05578	0.041481	0.007094	93.36	1.86	4.60	51	LP2 (2026-09-30)	PASS	NonPolyA_perM 51 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does