Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-22) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM4837369	M	M	NA	Agree	0.65	21.45	0.00	53.84	45.49	45.63	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.467563	0.462814	0.069524	0.000099	94.71	2.14	2.58	1866	LP2 (2026-09-30)	PASS	NonPolyA_perM 1866 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM4837370	M	M	NA	Agree	1.41	23.69	0.00	60.66	50.49	50.38	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.428668	0.468528	0.102739	0.000064	94.36	2.42	2.35	1903	LP2 (2026-09-30)	PASS	NonPolyA_perM 1903 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM4837371	M	M	NA	Agree	0.95	25.41	0.00	62.12	47.18	49.98	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.436148	0.461691	0.102088	0.000074	94.22	2.43	2.49	1846	LP2 (2026-09-30)	PASS	NonPolyA_perM 1846 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM4837372	M	M	NA	Agree	0.93	21.85	0.00	54.59	49.95	44.99	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.457123	0.458838	0.083969	0.000071	94.80	2.29	2.19	1800	LP2 (2026-09-30)	PASS	NonPolyA_perM 1800 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM4837373	M	M	NA	Agree	0.77	19.34	0.00	44.42	28.34	48.42	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.506024	0.412393	0.081459	0.000124	94.28	2.64	2.34	2572	LP2 (2026-09-30)	PASS	NonPolyA_perM 2572 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM4837374	M	M	NA	Agree	1.03	20.81	0.00	55.13	38.76	43.97	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.467323	0.443564	0.089005	0.000108	94.43	2.54	2.25	2439	LP2 (2026-09-30)	PASS	NonPolyA_perM 2439 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not