Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-20) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM4820173	M	M	NA	Agree	0.02	7.62	0.00	43.62	15.52	49.69	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.818886	0.109528	0.048886	0.0227	90.90	4.22	4.53	109	LP2 (2026-09-30)	PASS	NonPolyA_perM 109 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4820174	M	M	NA	Agree	0.01	8.64	0.00	37.33	14.12	54.08	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.830192	0.097261	0.049357	0.023191	92.00	4.31	3.38	96	LP2 (2026-09-30)	PASS	NonPolyA_perM 96 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4820175	M	M	NA	Agree	0.01	6.08	0.00	29.18	11.08	49.70	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.850172	0.087963	0.048846	0.013019	91.32	3.69	4.58	79	LP2 (2026-09-30)	PASS	NonPolyA_perM 79 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4820176	M	M	NA	Agree	0.02	6.76	0.00	38.46	14.62	49.36	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.812522	0.104868	0.049198	0.033412	91.01	5.30	3.35	88	LP2 (2026-09-30)	PASS	NonPolyA_perM 88 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4820177	M	Undetermined	NA	Conflict	30.63	2.36	0.00	13.05	5.20	16.56	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.843676	0.092487	0.047429	0.016408	90.22	3.71	5.71	94	LP2 (2026-09-30)	PASS	NonPolyA_perM 94 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4820178	M	M	NA	Agree	0.77	8.47	0.00	48.95	18.14	62.15	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.812309	0.113356	0.049447	0.024888	90.49	4.39	4.76	108	LP2 (2026-09-30)	PASS	NonPolyA_perM 108 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4820179	M	M	NA	Agree	0.16	7.02	0.00	42.03	14.67	54.08	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.826597	0.103366	0.049301	0.020737	91.74	4.16	3.75	103	LP2 (2026-09-30)	PASS	NonPolyA_perM 103 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4820180	M	M	NA	Agree	0.06	5.80	0.00	39.19	12.64	50.82	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.838762	0.090062	0.046474	0.024702	91.05	4.68	3.97	81	LP2 (2026-09-30)	PASS	NonPolyA_perM 81 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4820181	M	M	NA	Agree	0.03	5.78	0.00	38.01	14.15	52.63	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.832373	0.090689	0.047997	0.02894	90.27	5.00	4.39	96	LP2 (2026-09-30)	PASS	NonPolyA_perM 96 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4820182	M	M	NA	Agree	0.01	6.01	0.00	34.81	13.28	56.88	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.8397	0.085961	0.046448	0.02789	90.59	4.81	4.28	87	LP2 (2026-09-30)	PASS	NonPolyA_perM 87 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4820183	M	M	NA	Agree	0.04	5.79	0.00	35.52	12.09	47.76	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.842724	0.080983	0.047966	0.028327	89.12	4.94	5.55	73	LP2 (2026-09-30)	PASS	NonPolyA_perM 73 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4820184	M	M	NA	Agree	0.01	7.37	0.00	35.43	13.03	44.61	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.830133	0.093055	0.048702	0.02811	89.96	4.88	4.77	111	LP2 (2026-09-30)	PASS	NonPolyA_perM 111 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does