Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-20) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM4805004	F	F	NA	Agree	73.64	0.00	0.00	0.05	0.00	0.09	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.916374	0.055604	0.025729	0.002293	95.19	3.60	0.84	42	LP2 (2026-09-30)	PASS	NonPolyA_perM 42 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4805005	F	F	NA	Agree	91.02	0.00	0.00	0.08	0.00	0.08	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.912595	0.056351	0.025923	0.005131	94.45	3.87	1.31	50	LP2 (2026-09-30)	PASS	NonPolyA_perM 50 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4805006	F	F	NA	Agree	100.04	0.00	0.00	0.10	0.04	0.18	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.913213	0.058361	0.025758	0.002668	95.23	3.51	0.91	54	LP2 (2026-09-30)	PASS	NonPolyA_perM 54 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4805007	F	F	NA	Agree	93.41	0.00	0.00	0.00	0.04	0.00	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.905189	0.058077	0.026234	0.0105	94.48	4.34	0.80	54	LP2 (2026-09-30)	PASS	NonPolyA_perM 54 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4805008	F	M	NA	Conflict	9.62	3.71	0.00	22.92	5.70	37.79	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.925986	0.048111	0.023185	0.002718	95.84	3.20	0.62	46	LP2 (2026-09-30)	PASS	NonPolyA_perM 46 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4805009	F	F	NA	Agree	96.92	0.00	0.00	0.04	0.00	0.08	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.905538	0.062452	0.025832	0.006178	95.09	3.86	0.68	60	LP2 (2026-09-30)	PASS	NonPolyA_perM 60 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4805010	F	F	NA	Agree	89.38	0.00	0.00	0.00	0.00	0.00	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.920535	0.052221	0.024393	0.002851	95.43	3.35	0.85	44	LP2 (2026-09-30)	PASS	NonPolyA_perM 44 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4805011	F	F	NA	Agree	48.98	0.00	0.00	0.00	0.00	0.00	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.925768	0.046411	0.024643	0.003177	94.73	3.87	1.02	47	LP2 (2026-09-30)	PASS	NonPolyA_perM 47 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4805012	F	F	NA	Agree	111.60	0.00	0.00	0.00	0.00	0.00	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.915494	0.057581	0.025696	0.001229	95.53	3.29	0.81	55	LP2 (2026-09-30)	PASS	NonPolyA_perM 55 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4805013	F	F	NA	Agree	59.22	0.00	0.00	0.05	0.00	0.10	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.915254	0.058488	0.024313	0.001945	94.83	3.73	1.01	51	LP2 (2026-09-30)	PASS	NonPolyA_perM 51 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4805014	F	M	NA	Conflict	10.93	3.62	0.00	25.13	7.27	37.12	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.919835	0.053816	0.024256	0.002092	95.25	3.30	1.10	52	LP2 (2026-09-30)	PASS	NonPolyA_perM 52 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4805015	F	F	NA	Agree	87.80	0.00	0.00	0.00	0.00	0.10	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.918683	0.054964	0.024425	0.001927	95.51	3.36	0.77	47	LP2 (2026-09-30)	PASS	NonPolyA_perM 47 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4805016	F	F	NA	Agree	89.61	0.03	0.00	0.07	0.04	0.09	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.910339	0.061081	0.025297	0.003284	94.68	3.62	1.30	50	LP2 (2026-09-30)	PASS	NonPolyA_perM 50 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4805017	F	F	NA	Agree	91.56	0.19	0.00	0.40	0.19	1.17	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.905832	0.061313	0.026292	0.006563	94.28	4.10	1.22	60	LP2 (2026-09-30)	PASS	NonPolyA_perM 60 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4805018	F	F	NA	Agree	15.72	0.00	0.00	0.00	0.09	0.10	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.942505	0.03421	0.021013	0.002272	89.42	3.37	6.85	35	LP2 (2026-09-30)	PASS	NonPolyA_perM 35 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4805019	F	F	NA	Agree	85.08	0.00	0.00	0.00	0.02	0.08	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.913481	0.054719	0.025524	0.006275	94.44	3.98	1.21	44	LP2 (2026-09-30)	PASS	NonPolyA_perM 44 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4805020	F	M	NA	Conflict	6.01	5.70	0.00	31.99	8.37	52.20	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.93406	0.040557	0.024012	0.001371	95.56	3.06	1.03	33	LP2 (2026-09-30)	PASS	NonPolyA_perM 33 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4805021	F	F	NA	Agree	82.68	0.00	0.00	0.10	0.06	0.09	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.918545	0.053323	0.024267	0.003866	95.15	3.63	0.85	47	LP2 (2026-09-30)	PASS	NonPolyA_perM 47 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4805022	F	F	NA	Agree	63.74	0.00	0.00	0.00	0.00	0.00	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.92308	0.050204	0.024539	0.002178	95.13	3.53	0.95	42	LP2 (2026-09-30)	PASS	NonPolyA_perM 42 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4805023	F	F	NA	Agree	99.79	0.00	0.00	0.00	0.00	0.00	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.913081	0.059969	0.025314	0.001637	95.17	3.33	1.14	53	LP2 (2026-09-30)	PASS	NonPolyA_perM 53 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4805024	F	F	NA	Agree	94.04	0.00	0.00	0.04	0.00	0.00	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.914859	0.057041	0.024872	0.003227	95.38	3.38	0.87	46	LP2 (2026-09-30)	PASS	NonPolyA_perM 46 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4805025	F	F	NA	Agree	42.55	0.00	0.00	0.06	0.05	0.23	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.930654	0.042111	0.024114	0.003122	94.07	3.82	1.73	44	LP2 (2026-09-30)	PASS	NonPolyA_perM 44 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4805026	F	M	NA	Conflict	4.23	5.20	0.00	28.57	7.44	50.83	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.922933	0.051222	0.024485	0.00136	94.15	3.12	2.38	50	LP2 (2026-09-30)	PASS	NonPolyA_perM 50 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4805027	F	F	NA	Agree	60.15	0.02	0.00	0.05	0.05	0.29	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.923617	0.049625	0.024284	0.002474	95.07	3.61	0.93	36	LP2 (2026-09-30)	PASS	NonPolyA_perM 36 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4805028	M	M	NA	Agree	0.14	3.85	0.00	25.76	7.52	50.39	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.928371	0.04675	0.022814	0.002065	95.18	3.14	1.32	46	LP2 (2026-09-30)	PASS	NonPolyA_perM 46 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4805029	M	M	NA	Agree	0.23	4.80	0.00	27.35	7.59	51.87	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.917501	0.052729	0.023993	0.005777	94.87	3.60	1.17	52	LP2 (2026-09-30)	PASS	NonPolyA_perM 52 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4805030	M	M	NA	Agree	0.04	4.51	0.00	26.85	8.32	50.39	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.911112	0.058039	0.025058	0.005792	94.93	3.67	1.04	49	LP2 (2026-09-30)	PASS	NonPolyA_perM 49 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4805031	M	M	NA	Agree	0.01	3.96	0.00	29.48	7.13	52.47	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.92229	0.051046	0.024289	0.002375	95.56	3.33	0.77	53	LP2 (2026-09-30)	PASS	NonPolyA_perM 53 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4805032	M	F	NA	Conflict	78.73	0.30	0.00	1.78	0.46	3.72	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.925415	0.049424	0.023591	0.00157	95.68	3.15	0.84	44	LP2 (2026-09-30)	PASS	NonPolyA_perM 44 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4805033	M	M	NA	Agree	0.04	4.77	0.00	30.68	8.84	52.64	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.923146	0.051079	0.023994	0.00178	95.64	3.27	0.76	47	LP2 (2026-09-30)	PASS	NonPolyA_perM 47 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4805034	M	M	NA	Agree	0.27	3.16	0.00	22.48	6.79	42.25	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.912546	0.051255	0.023296	0.012902	93.94	4.26	1.45	49	LP2 (2026-09-30)	PASS	NonPolyA_perM 49 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4805035	M	M	NA	Agree	0.22	4.22	0.00	27.64	7.20	44.95	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.923266	0.050302	0.023696	0.002736	94.91	3.33	1.40	55	LP2 (2026-09-30)	PASS	NonPolyA_perM 55 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4805036	M	M	NA	Agree	0.24	5.46	0.00	32.71	7.54	48.04	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.921159	0.052495	0.023533	0.002813	95.29	3.26	1.09	49	LP2 (2026-09-30)	PASS	NonPolyA_perM 49 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4805037	M	M	NA	Agree	0.43	3.90	0.00	28.08	7.92	48.04	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.924697	0.048568	0.024	0.002735	94.84	3.30	1.51	43	LP2 (2026-09-30)	PASS	NonPolyA_perM 43 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4805038	M	F	NA	Conflict	83.02	0.32	0.00	3.19	0.82	6.96	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.917175	0.054515	0.025402	0.002908	94.99	3.47	1.17	55	LP2 (2026-09-30)	PASS	NonPolyA_perM 55 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4805039	M	M	NA	Agree	0.04	4.48	0.00	23.52	5.54	46.09	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.947268	0.027908	0.020699	0.004126	95.26	3.43	0.97	22	LP2 (2026-09-30)	PASS	NonPolyA_perM 22 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4805040	M	M	NA	Agree	0.05	4.57	0.00	24.76	8.08	47.37	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.913899	0.059044	0.024751	0.002306	94.78	3.26	1.60	47	LP2 (2026-09-30)	PASS	NonPolyA_perM 47 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4805041	M	M	NA	Agree	0.11	3.13	0.00	24.36	7.49	46.17	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.921445	0.05196	0.024764	0.001831	95.40	3.36	0.89	43	LP2 (2026-09-30)	PASS	NonPolyA_perM 43 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4805042	M	M	NA	Agree	1.08	1.92	0.00	12.99	4.23	36.01	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.932676	0.041836	0.023302	0.002186	95.18	3.47	1.01	45	LP2 (2026-09-30)	PASS	NonPolyA_perM 45 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4805043	M	M	NA	Agree	4.03	4.01	0.00	26.28	7.32	45.42	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.920137	0.053175	0.024537	0.002152	95.35	3.43	0.85	53	LP2 (2026-09-30)	PASS	NonPolyA_perM 53 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4805044	M	F	NA	Conflict	71.43	0.48	0.00	2.43	0.61	5.22	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.922215	0.052656	0.023765	0.001363	95.64	3.15	0.87	38	LP2 (2026-09-30)	PASS	NonPolyA_perM 38 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4805045	M	M	NA	Agree	0.06	4.07	0.00	26.66	7.45	46.18	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.908372	0.060082	0.024787	0.006758	94.84	3.81	0.97	60	LP2 (2026-09-30)	PASS	NonPolyA_perM 60 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4805046	M	M	NA	Agree	0.06	3.83	0.00	28.35	8.23	45.29	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.921887	0.051266	0.023637	0.00321	95.23	3.30	1.10	48	LP2 (2026-09-30)	PASS	NonPolyA_perM 48 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4805047	M	M	NA	Agree	0.11	3.95	0.00	25.55	7.08	51.26	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.918158	0.055393	0.024366	0.002083	95.48	3.26	0.88	54	LP2 (2026-09-30)	PASS	NonPolyA_perM 54 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4805048	M	M	NA	Agree	0.08	3.21	0.00	18.38	7.04	46.04	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.920751	0.052916	0.023781	0.002552	95.46	3.26	0.92	49	LP2 (2026-09-30)	PASS	NonPolyA_perM 49 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4805049	M	M	NA	Agree	0.06	4.65	0.00	24.25	6.66	38.93	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.923346	0.050791	0.023467	0.002396	95.37	3.26	1.00	44	LP2 (2026-09-30)	PASS	NonPolyA_perM 44 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4805050	M	F	NA	Conflict	89.34	0.34	0.00	2.54	0.60	3.83	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.911153	0.057948	0.025824	0.005075	94.93	3.74	0.96	52	LP2 (2026-09-30)	PASS	NonPolyA_perM 52 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4805051	M	M	NA	Agree	0.09	5.12	0.00	30.46	8.04	52.02	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.928434	0.046568	0.022564	0.002434	95.61	3.12	0.90	43	LP2 (2026-09-30)	PASS	NonPolyA_perM 43 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does