Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-21) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM4715917	M	M	NA	Agree	0.00	8.58	0.00	42.98	24.01	55.40	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.872968	0.082179	0.042333	0.002521	90.02	3.16	6.03	117	LP2 (2026-09-30)	PASS	NonPolyA_perM 117 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4715918	M	M	NA	Agree	0.00	6.59	0.00	43.55	19.61	55.88	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.870474	0.086043	0.040725	0.002758	90.13	3.18	5.86	123	LP2 (2026-09-30)	PASS	NonPolyA_perM 123 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4715919	M	M	NA	Agree	0.02	6.55	0.00	39.85	20.55	55.32	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.86674	0.09022	0.04143	0.00161	91.15	3.19	4.78	125	LP2 (2026-09-30)	PASS	NonPolyA_perM 125 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4715920	M	M	NA	Agree	0.01	7.80	0.00	45.31	23.09	62.08	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.867414	0.087268	0.041956	0.003362	91.76	3.21	4.18	110	LP2 (2026-09-30)	PASS	NonPolyA_perM 110 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4715921	M	M	NA	Agree	0.02	6.74	0.00	40.34	23.28	57.39	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.871725	0.081681	0.043793	0.002801	89.17	3.22	6.76	123	LP2 (2026-09-30)	PASS	NonPolyA_perM 123 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4715922	M	M	NA	Agree	0.01	8.49	0.00	47.32	23.01	59.83	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.864503	0.089356	0.042121	0.00402	90.92	3.25	5.02	116	LP2 (2026-09-30)	PASS	NonPolyA_perM 116 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4715923	M	M	NA	Agree	0.36	7.25	0.00	36.51	20.44	57.49	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.869936	0.087716	0.040845	0.001503	88.82	3.18	7.24	135	LP2 (2026-09-30)	PASS	NonPolyA_perM 135 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4715924	M	M	NA	Agree	0.00	6.10	0.00	35.99	18.09	58.31	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.854794	0.089246	0.040145	0.015815	89.67	4.60	4.96	148	LP2 (2026-09-30)	PASS	NonPolyA_perM 148 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4715925	M	M	NA	Agree	0.15	6.42	0.00	36.70	18.61	57.59	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.875371	0.083794	0.038173	0.002662	91.20	3.31	4.76	112	LP2 (2026-09-30)	PASS	NonPolyA_perM 112 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4715926	M	M	NA	Agree	0.00	6.46	0.00	33.92	18.19	53.84	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.865598	0.080948	0.037633	0.015822	90.43	4.50	4.44	137	LP2 (2026-09-30)	PASS	NonPolyA_perM 137 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4715927	M	M	NA	Agree	0.03	6.49	0.00	29.92	17.41	57.65	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.867582	0.087053	0.03807	0.007295	91.37	3.75	4.13	128	LP2 (2026-09-30)	PASS	NonPolyA_perM 128 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4715928	M	M	NA	Agree	0.10	5.55	0.00	32.62	16.06	56.32	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.886398	0.074588	0.037344	0.00167	90.26	3.25	5.82	114	LP2 (2026-09-30)	PASS	NonPolyA_perM 114 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4715929	M	M	NA	Agree	0.00	9.06	0.00	42.23	21.20	60.03	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.842925	0.096031	0.040936	0.020108	88.36	4.80	6.09	153	LP2 (2026-09-30)	PASS	NonPolyA_perM 153 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4715930	M	M	NA	Agree	0.01	6.08	0.00	39.99	19.05	53.44	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.809931	0.082934	0.039647	0.067488	83.55	9.39	6.29	197	LP2 (2026-09-30)	PASS	NonPolyA_perM 197 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4715931	M	M	NA	Agree	0.03	6.46	0.00	40.66	19.84	56.69	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.880497	0.077	0.039683	0.00282	90.29	3.15	5.82	111	LP2 (2026-09-30)	PASS	NonPolyA_perM 111 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4715932	M	M	NA	Agree	0.03	6.52	0.00	44.36	22.67	61.54	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.871031	0.083371	0.040357	0.005241	90.59	3.42	5.18	107	LP2 (2026-09-30)	PASS	NonPolyA_perM 107 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4715933	M	M	NA	Agree	0.00	7.71	0.00	43.48	21.75	64.73	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.8607	0.094962	0.042235	0.002103	91.86	3.13	4.18	116	LP2 (2026-09-30)	PASS	NonPolyA_perM 116 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4715934	M	M	NA	Agree	0.00	7.77	0.00	50.34	24.25	53.56	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.870309	0.085923	0.04245	0.001317	90.30	3.04	5.94	117	LP2 (2026-09-30)	PASS	NonPolyA_perM 117 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4715935	M	M	NA	Agree	0.03	6.71	0.00	32.96	18.20	55.15	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.872107	0.086599	0.038541	0.002753	91.43	3.34	4.53	126	LP2 (2026-09-30)	PASS	NonPolyA_perM 126 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4715936	M	M	NA	Agree	0.01	6.62	0.00	35.81	20.04	53.54	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.86808	0.088106	0.039518	0.004296	87.96	3.48	7.90	112	LP2 (2026-09-30)	PASS	NonPolyA_perM 112 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4715937	M	M	NA	Agree	0.00	6.28	0.00	34.40	17.46	55.87	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.864824	0.091914	0.039116	0.004147	88.87	3.49	6.88	134	LP2 (2026-09-30)	PASS	NonPolyA_perM 134 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4715938	M	M	NA	Agree	0.00	5.82	0.00	30.25	18.60	56.01	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.830023	0.082728	0.038204	0.049045	81.49	7.73	10.19	149	LP2 (2026-09-30)	PASS	NonPolyA_perM 149 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4715939	M	M	NA	Agree	0.27	6.56	0.00	34.83	17.09	59.46	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.871751	0.082656	0.04176	0.003833	90.17	3.65	5.14	124	LP2 (2026-09-30)	PASS	NonPolyA_perM 124 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4715940	M	M	NA	Agree	0.04	5.23	0.00	37.41	21.57	46.35	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.869772	0.084818	0.039617	0.005793	86.98	3.59	8.79	114	LP2 (2026-09-30)	PASS	NonPolyA_perM 114 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does