Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-21) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM4495557	M	M	NA	Agree	0.00	5.55	0.00	36.55	19.24	46.67	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.882587	0.074218	0.041823	0.001372	64.21	2.00	33.23	80	LP2 (2026-09-30)	PASS	NonPolyA_perM 80 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4495558	M	M	NA	Agree	0.00	5.98	0.00	37.70	20.67	48.97	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.884131	0.0707	0.043473	0.001695	63.59	2.09	33.74	80	LP2 (2026-09-30)	PASS	NonPolyA_perM 80 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4495559	M	M	NA	Agree	0.00	5.45	0.00	37.32	18.36	48.64	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.883095	0.073631	0.041805	0.00147	64.22	2.05	33.16	101	LP2 (2026-09-30)	PASS	NonPolyA_perM 101 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4495560	M	M	NA	Agree	0.00	6.34	0.00	35.55	19.66	46.11	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.881843	0.072298	0.043686	0.002173	68.18	2.25	28.96	98	LP2 (2026-09-30)	PASS	NonPolyA_perM 98 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4495561	M	M	NA	Agree	0.05	6.15	0.00	35.99	17.52	54.00	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.873649	0.082145	0.043133	0.001072	66.40	2.08	30.93	86	LP2 (2026-09-30)	PASS	NonPolyA_perM 86 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4495562	M	M	NA	Agree	0.00	7.18	0.00	37.34	16.92	51.88	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.882524	0.074917	0.041816	0.000743	66.49	2.01	30.93	78	LP2 (2026-09-30)	PASS	NonPolyA_perM 78 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4495563	M	M	NA	Agree	0.05	4.48	0.00	30.60	17.26	50.37	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.878743	0.077876	0.041188	0.002193	66.22	2.17	31.06	102	LP2 (2026-09-30)	PASS	NonPolyA_perM 102 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4495564	M	M	NA	Agree	0.00	4.86	0.00	25.94	15.40	48.95	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.891134	0.069116	0.037632	0.002117	72.13	2.25	25.10	104	LP2 (2026-09-30)	PASS	NonPolyA_perM 104 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4495565	M	M	NA	Agree	0.07	6.77	0.00	27.54	16.82	44.85	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.890352	0.069627	0.038749	0.001271	60.91	1.94	36.67	92	LP2 (2026-09-30)	PASS	NonPolyA_perM 92 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4495566	M	M	NA	Agree	0.04	5.20	0.00	32.80	19.97	48.22	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.879644	0.077302	0.041388	0.001666	62.79	2.05	34.64	98	LP2 (2026-09-30)	PASS	NonPolyA_perM 98 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4495567	M	M	NA	Agree	0.04	7.03	0.00	30.54	13.75	46.84	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.889049	0.068291	0.039163	0.003497	59.90	2.15	37.47	90	LP2 (2026-09-30)	PASS	NonPolyA_perM 90 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4495568	M	M	NA	Agree	0.03	4.57	0.00	28.22	16.27	45.16	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.887456	0.06945	0.039607	0.003487	57.07	2.06	40.44	111	LP2 (2026-09-30)	PASS	NonPolyA_perM 111 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4495569	M	M	NA	Agree	0.00	5.52	0.00	31.15	18.08	44.94	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.88922	0.067952	0.040611	0.002217	64.13	2.02	33.32	73	LP2 (2026-09-30)	PASS	NonPolyA_perM 73 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4495570	M	M	NA	Agree	0.03	4.80	0.00	30.52	18.26	42.80	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.878961	0.071685	0.041738	0.007616	57.56	2.33	39.63	94	LP2 (2026-09-30)	PASS	NonPolyA_perM 94 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4495571	M	M	NA	Agree	0.00	6.58	0.00	31.18	17.75	45.85	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.780667	0.071247	0.037718	0.110368	51.47	10.69	37.37	211	LP2 (2026-09-30)	PASS	NonPolyA_perM 211 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4495572	M	M	NA	Agree	0.00	4.66	0.00	26.05	18.56	42.60	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.886285	0.069153	0.04084	0.003722	62.62	2.13	34.77	74	LP2 (2026-09-30)	PASS	NonPolyA_perM 74 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4495573	M	M	NA	Agree	0.00	7.31	0.00	38.32	22.41	50.74	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.868831	0.08551	0.043419	0.00224	69.62	2.24	27.53	82	LP2 (2026-09-30)	PASS	NonPolyA_perM 82 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4495574	M	M	NA	Agree	0.00	4.85	0.00	32.75	19.48	45.20	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.884291	0.072297	0.042306	0.001106	69.09	2.09	28.25	80	LP2 (2026-09-30)	PASS	NonPolyA_perM 80 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4495575	M	M	NA	Agree	0.09	7.31	0.00	34.53	17.80	50.15	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.871542	0.073164	0.040372	0.014923	72.03	3.54	23.85	96	LP2 (2026-09-30)	PASS	NonPolyA_perM 96 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4495576	M	M	NA	Agree	0.61	5.80	0.00	35.35	18.56	54.32	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.876747	0.077705	0.041851	0.003697	69.74	2.48	27.15	87	LP2 (2026-09-30)	PASS	NonPolyA_perM 87 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4495577	M	M	NA	Agree	0.12	4.83	0.00	24.63	17.56	48.80	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.86129	0.069846	0.038502	0.030362	71.60	5.01	22.88	110	LP2 (2026-09-30)	PASS	NonPolyA_perM 110 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4495578	M	M	NA	Agree	0.31	6.57	0.00	33.48	17.77	48.62	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.85783	0.079961	0.038536	0.023673	73.71	4.38	21.37	128	LP2 (2026-09-30)	PASS	NonPolyA_perM 128 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4495579	M	M	NA	Agree	0.06	6.98	0.00	33.53	1.08	71.65	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.820022	0.019935	0.021919	0.138123	59.74	15.24	24.78	226	LP2 (2026-09-30)	PASS	NonPolyA_perM 226 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4495580	M	M	NA	Agree	0.10	6.28	0.00	32.93	18.06	45.85	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.875809	0.07921	0.039357	0.005624	72.04	2.61	24.80	94	LP2 (2026-09-30)	PASS	NonPolyA_perM 94 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does