Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-22) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM4487275	M	M	NA	Agree	0.03	9.24	0.00	32.84	14.09	97.08	NONE	POLYA	0.848007	0.082792	0.050317	0.018884	92.11	3.54	4.03	74	LP2 (2026-09-30)	PASS	NonPolyA_perM 74 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4487276	M	M	NA	Agree	0.07	5.91	0.00	22.55	10.72	87.92	NONE	POLYA	0.871174	0.074723	0.044722	0.00938	93.87	2.73	3.06	54	LP2 (2026-09-30)	PASS	NonPolyA_perM 54 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4487277	M	M	NA	Agree	0.04	7.07	0.00	28.02	10.35	85.73	NONE	POLYA	0.862703	0.072324	0.04612	0.018853	92.15	3.63	3.88	55	LP2 (2026-09-30)	PASS	NonPolyA_perM 55 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4487278	M	M	NA	Agree	0.05	4.37	0.00	21.15	10.02	76.37	NONE	POLYA	0.883402	0.065866	0.039951	0.010781	93.83	2.91	3.00	51	LP2 (2026-09-30)	PASS	NonPolyA_perM 51 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4487279	M	M	NA	Agree	0.05	5.50	0.00	23.70	11.22	78.23	NONE	POLYA	0.816559	0.08326	0.042926	0.057256	89.30	7.19	3.22	74	LP2 (2026-09-30)	PASS	NonPolyA_perM 74 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4487280	M	M	NA	Agree	0.07	5.52	0.00	22.89	11.05	84.34	NONE	POLYA	0.864621	0.074418	0.04482	0.016141	92.68	3.37	3.64	60	LP2 (2026-09-30)	PASS	NonPolyA_perM 60 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does