Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-22) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM4474715	M	M	NA	Agree	0.03	4.84	0.00	42.11	8.53	40.90	NONE	POLYA	0.844856	0.105031	0.037181	0.012932	92.50	3.75	3.45	324	LP2 (2026-09-30)	PASS	NonPolyA_perM 324 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4474716	M	M	NA	Agree	0.05	5.35	0.00	42.79	7.59	39.08	NONE	POLYA	0.82692	0.126194	0.034232	0.012654	92.56	3.72	3.44	346	LP2 (2026-09-30)	PASS	NonPolyA_perM 346 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4474717	M	M	NA	Agree	0.06	5.02	0.00	34.53	7.44	34.58	NONE	POLYA	0.831712	0.121124	0.035976	0.011188	92.70	3.63	3.39	284	LP2 (2026-09-30)	PASS	NonPolyA_perM 284 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4474718	M	M	NA	Agree	0.04	7.29	0.00	55.11	7.38	40.32	NONE	POLYA	0.865414	0.091959	0.032828	0.009798	92.42	3.60	3.67	197	LP2 (2026-09-30)	PASS	NonPolyA_perM 197 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4474719	M	M	NA	Agree	0.06	4.85	0.00	42.12	7.47	31.23	NONE	POLYA	0.85761	0.095792	0.033279	0.01332	92.30	3.69	3.70	178	LP2 (2026-09-30)	PASS	NonPolyA_perM 178 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4474720	M	M	NA	Agree	0.09	8.26	0.00	67.29	7.38	43.23	NONE	POLYA	0.846349	0.115127	0.032168	0.006356	93.41	2.82	3.44	221	LP2 (2026-09-30)	PASS	NonPolyA_perM 221 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4474721	M	M	NA	Agree	0.06	10.38	0.00	92.98	16.94	46.54	NONE	POLYA	0.759076	0.171626	0.056966	0.012332	92.01	3.63	3.96	329	LP2 (2026-09-30)	PASS	NonPolyA_perM 329 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4474722	M	M	NA	Agree	0.09	9.42	0.00	95.13	13.45	53.38	NONE	POLYA	0.821122	0.132228	0.036341	0.010309	92.53	3.51	3.69	259	LP2 (2026-09-30)	PASS	NonPolyA_perM 259 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4474723	M	M	NA	Agree	0.06	6.64	0.00	54.56	8.88	40.41	NONE	POLYA	0.777259	0.151123	0.060125	0.011494	92.01	3.83	3.78	344	LP2 (2026-09-30)	PASS	NonPolyA_perM 344 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does