Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-20) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO). SampleID InputSex ComputedSex XYRatio Agreement Xist Uty Sry Ddx3y Kdm5d Eif2s3y ComputedStrand ComputedLibraryPrep PctMRNA PctIntronic PctIntergenic PctRibosomal UniqPct MultiPct TooShortPct NonPolyA_perM LibraryPrepRule LibraryPrepQC LibraryPrepReason GSM4447250 F F NA Agree 219.00 0.00 0.00 0.00 0.00 0.00 NONE POLYA 0.882615 0.072913 0.038505 0.005967 94.90 3.49 1.11 106 LP2 (2026-09-30) PASS NonPolyA_perM 106 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM4447251 F F NA Agree 123.42 0.00 0.00 0.00 0.00 0.00 NONE POLYA 0.90473 0.053823 0.036262 0.005185 92.62 5.85 1.07 130 LP2 (2026-09-30) PASS NonPolyA_perM 130 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM4447252 F F NA Agree 56.21 0.00 0.00 0.00 0.00 0.00 NONE POLYA 0.928399 0.032317 0.035308 0.003975 95.00 3.59 1.01 65 LP2 (2026-09-30) PASS NonPolyA_perM 65 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM4447253 F F NA Agree 60.49 0.00 0.00 0.00 0.00 0.00 NONE POLYA 0.926107 0.032214 0.036691 0.004988 94.89 3.60 1.08 56 LP2 (2026-09-30) PASS NonPolyA_perM 56 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM4447254 F F NA Agree 226.12 0.00 0.00 0.00 0.00 0.00 NONE POLYA 0.883293 0.074194 0.038744 0.003769 95.03 3.60 1.04 109 LP2 (2026-09-30) PASS NonPolyA_perM 109 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM4447255 F F NA Agree 178.08 0.00 0.00 0.00 0.00 0.00 NONE POLYA 0.901605 0.056577 0.038243 0.003575 93.92 4.27 1.29 108 LP2 (2026-09-30) PASS NonPolyA_perM 108 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM4447256 F F NA Agree 197.09 0.00 0.00 0.00 0.00 0.00 NONE POLYA 0.777241 0.077069 0.03799 0.1077 86.04 12.61 0.96 102 LP2 (2026-09-30) PASS NonPolyA_perM 102 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM4447257 F F NA Agree 257.77 0.00 0.00 0.00 0.00 0.00 NONE POLYA 0.865693 0.087523 0.039708 0.007076 95.76 3.04 0.86 84 LP2 (2026-09-30) PASS NonPolyA_perM 84 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM4447258 F F NA Agree 227.56 0.00 0.00 0.00 0.00 0.00 NONE POLYA 0.903467 0.053223 0.037237 0.006074 95.65 3.15 0.92 66 LP2 (2026-09-30) PASS NonPolyA_perM 66 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM4447259 F F NA Agree 191.70 0.00 0.00 0.00 0.00 0.00 NONE POLYA 0.918319 0.038403 0.038771 0.004507 95.65 3.14 0.92 51 LP2 (2026-09-30) PASS NonPolyA_perM 51 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM4447260 F F NA Agree 163.76 0.00 0.00 0.00 0.00 0.00 NONE POLYA 0.911548 0.042597 0.040578 0.005277 95.23 3.35 1.06 69 LP2 (2026-09-30) PASS NonPolyA_perM 69 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM4447261 F F NA Agree 74.83 0.00 0.00 0.00 0.00 0.00 NONE POLYA 0.924895 0.035233 0.034633 0.005239 91.66 6.76 1.02 71 LP2 (2026-09-30) PASS NonPolyA_perM 71 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM4447262 F F NA Agree 311.64 0.00 0.00 0.00 0.00 0.00 NONE POLYA 0.878593 0.06852 0.044356 0.008531 95.18 3.32 1.10 65 LP2 (2026-09-30) PASS NonPolyA_perM 65 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM4447263 F F NA Agree 285.93 0.00 0.00 0.00 0.00 0.00 NONE POLYA 0.881585 0.069318 0.043113 0.005984 95.35 3.14 1.11 72 LP2 (2026-09-30) PASS NonPolyA_perM 72 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM4447264 F F NA Agree 321.91 0.00 0.00 0.00 0.00 0.00 NONE POLYA 0.878258 0.074072 0.042752 0.004918 95.56 3.10 0.92 56 LP2 (2026-09-30) PASS NonPolyA_perM 56 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM4447265 F F NA Agree 409.45 0.00 0.00 0.00 0.00 0.00 NONE POLYA 0.839893 0.10971 0.04645 0.003947 95.71 2.74 1.13 99 LP2 (2026-09-30) PASS NonPolyA_perM 99 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM4447266 F F NA Agree 237.60 0.00 0.00 0.00 0.00 0.00 NONE POLYA 0.864194 0.087319 0.041122 0.007365 95.23 3.29 1.01 107 LP2 (2026-09-30) PASS NonPolyA_perM 107 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM4447267 F F NA Agree 123.61 0.00 0.00 0.00 0.00 0.00 NONE POLYA 0.914704 0.041532 0.038838 0.004926 95.37 3.28 0.94 57 LP2 (2026-09-30) PASS NonPolyA_perM 57 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM4447268 F F NA Agree 134.98 0.00 0.00 0.00 0.00 0.00 NONE POLYA 0.915286 0.039864 0.039817 0.005033 94.79 3.26 1.48 47 LP2 (2026-09-30) PASS NonPolyA_perM 47 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM4447269 F F NA Agree 85.88 0.00 0.00 0.00 0.00 0.00 NONE POLYA 0.918993 0.043599 0.033887 0.003521 95.40 3.11 1.08 65 LP2 (2026-09-30) PASS NonPolyA_perM 65 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM4447270 F F NA Agree 184.46 0.00 0.00 0.00 0.00 0.00 NONE POLYA 0.907749 0.048367 0.037316 0.006568 95.55 3.09 1.01 57 LP2 (2026-09-30) PASS NonPolyA_perM 57 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM4447271 F F NA Agree 53.77 0.00 0.00 0.00 0.00 0.00 NONE POLYA 0.927555 0.03492 0.034176 0.003349 95.70 2.92 1.02 35 LP2 (2026-09-30) PASS NonPolyA_perM 35 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM4447272 F F NA Agree 115.28 0.00 0.00 0.00 0.00 0.00 NONE POLYA 0.900676 0.047864 0.043798 0.007662 94.90 3.68 1.05 77 LP2 (2026-09-30) PASS NonPolyA_perM 77 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM4447273 F F NA Agree 106.14 0.00 0.00 0.00 0.00 0.00 NONE POLYA 0.917461 0.037543 0.040156 0.00484 95.39 3.24 1.01 43 LP2 (2026-09-30) PASS NonPolyA_perM 43 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM4447274 F F NA Agree 112.58 0.00 0.00 0.00 0.00 0.00 NONE POLYA 0.906555 0.0381 0.048286 0.007059 94.79 3.67 1.10 45 LP2 (2026-09-30) PASS NonPolyA_perM 45 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM4447275 F F NA Agree 311.64 0.00 0.00 0.00 0.00 0.00 NONE POLYA 0.871391 0.078762 0.041363 0.008484 95.06 3.40 1.10 94 LP2 (2026-09-30) PASS NonPolyA_perM 94 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM4447276 F F NA Agree 339.58 0.00 0.00 0.00 0.00 0.00 NONE POLYA 0.865382 0.087256 0.040921 0.006441 95.45 3.13 0.96 121 LP2 (2026-09-30) PASS NonPolyA_perM 121 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM4447277 F F NA Agree 202.11 0.00 0.00 0.00 0.00 0.00 NONE POLYA 0.879634 0.071483 0.040563 0.008321 95.18 3.36 1.06 85 LP2 (2026-09-30) PASS NonPolyA_perM 85 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM4447278 F F NA Agree 183.50 0.00 0.00 0.00 0.00 0.00 NONE POLYA 0.916989 0.041774 0.036891 0.004347 95.44 3.13 1.10 55 LP2 (2026-09-30) PASS NonPolyA_perM 55 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM4447279 F F NA Agree 45.66 0.00 0.00 0.00 0.00 0.00 NONE POLYA 0.922089 0.036455 0.038461 0.002995 95.11 3.28 1.19 46 LP2 (2026-09-30) PASS NonPolyA_perM 46 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM4447280 F F NA Agree 208.95 0.00 0.00 0.00 0.00 0.00 NONE POLYA 0.900977 0.052208 0.041276 0.005539 95.21 3.32 1.10 100 LP2 (2026-09-30) PASS NonPolyA_perM 100 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM4447281 F F NA Agree 199.06 0.00 0.00 0.00 0.00 0.00 NONE POLYA 0.879106 0.073209 0.041574 0.006111 95.71 3.04 0.95 81 LP2 (2026-09-30) PASS NonPolyA_perM 81 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM4447282 F F NA Agree 221.99 0.00 0.00 0.00 0.00 0.00 NONE POLYA 0.879299 0.071527 0.042794 0.006381 95.56 3.08 1.03 97 LP2 (2026-09-30) PASS NonPolyA_perM 97 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM4447283 F F NA Agree 204.21 0.00 0.00 0.00 0.00 0.00 NONE POLYA 0.874566 0.076749 0.042981 0.005704 95.11 3.31 1.17 83 LP2 (2026-09-30) PASS NonPolyA_perM 83 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM4447284 F F NA Agree 233.13 0.00 0.00 0.00 0.00 0.00 NONE POLYA 0.886716 0.066539 0.042636 0.004109 95.52 2.94 1.19 78 LP2 (2026-09-30) PASS NonPolyA_perM 78 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM4447285 F F NA Agree 57.19 0.00 0.00 0.00 0.00 0.00 NONE POLYA 0.918963 0.039705 0.038694 0.002638 93.83 3.93 1.87 99 LP2 (2026-09-30) PASS NonPolyA_perM 99 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM4447286 F F NA Agree 147.89 0.00 0.00 0.00 0.00 0.00 NONE POLYA 0.873387 0.077849 0.047962 0.000803 94.56 3.45 1.58 93 LP2 (2026-09-30) PASS NonPolyA_perM 93 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM4447287 F F NA Agree 99.24 0.00 0.00 0.00 0.00 0.00 NONE POLYA 0.872859 0.07162 0.053593 0.001928 93.78 3.79 1.98 90 LP2 (2026-09-30) PASS NonPolyA_perM 90 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM4447288 F F NA Agree 124.54 0.00 0.00 0.00 0.00 0.00 NONE POLYA 0.830999 0.10662 0.05766 0.004721 93.57 3.88 2.05 166 LP2 (2026-09-30) PASS NonPolyA_perM 166 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM4447289 F F NA Agree 121.16 0.00 0.00 0.00 0.00 0.00 NONE POLYA 0.885968 0.065492 0.045682 0.002858 94.18 3.47 1.94 92 LP2 (2026-09-30) PASS NonPolyA_perM 92 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM4447290 F F NA Agree 126.58 0.00 0.00 0.00 0.00 0.00 NONE POLYA 0.795833 0.122907 0.069987 0.011274 92.76 4.80 1.90 163 LP2 (2026-09-30) PASS NonPolyA_perM 163 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM4447291 F F NA Agree 144.50 0.00 0.00 0.00 0.00 0.00 NONE POLYA 0.874196 0.060307 0.062835 0.002662 89.61 5.46 1.88 125 LP2 (2026-09-30) PASS NonPolyA_perM 125 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM4447292 F F NA Agree 103.77 0.00 0.00 0.00 0.00 0.00 NONE POLYA 0.870123 0.062155 0.064838 0.002884 90.50 5.38 1.26 121 LP2 (2026-09-30) PASS NonPolyA_perM 121 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM4447293 F F NA Agree 136.92 0.00 0.00 0.00 0.00 0.00 NONE POLYA 0.865991 0.080558 0.049141 0.00431 93.66 3.90 1.94 142 LP2 (2026-09-30) PASS NonPolyA_perM 142 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM4447294 F F NA Agree 185.62 0.00 0.00 0.00 0.00 0.00 NONE POLYA 0.786069 0.13447 0.072379 0.007082 93.54 3.97 1.90 159 LP2 (2026-09-30) PASS NonPolyA_perM 159 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM4447295 F F NA Agree 211.58 0.00 0.00 0.00 0.00 0.00 NONE POLYA 0.849477 0.095396 0.051981 0.003145 94.10 3.52 1.94 107 LP2 (2026-09-30) PASS NonPolyA_perM 107 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM4447296 F F NA Agree 89.59 0.00 0.00 0.00 0.00 0.00 NONE POLYA 0.847096 0.086486 0.061703 0.004714 94.00 3.90 1.62 107 LP2 (2026-09-30) PASS NonPolyA_perM 107 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM4447297 F F NA Agree 60.82 0.00 0.00 0.00 0.00 0.00 NONE POLYA 0.885892 0.054469 0.056638 0.003 94.18 3.64 1.58 70 LP2 (2026-09-30) PASS NonPolyA_perM 70 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM4447298 F F NA Agree 193.72 0.00 0.00 0.00 0.00 0.00 NONE POLYA 0.797553 0.126386 0.067547 0.008514 93.44 4.15 1.85 243 LP2 (2026-09-30) PASS NonPolyA_perM 243 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM4447299 F F NA Agree 131.91 0.00 0.00 0.00 0.00 0.00 NONE POLYA 0.896324 0.050789 0.051028 0.001859 94.53 3.25 1.86 73 LP2 (2026-09-30) PASS NonPolyA_perM 73 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM4447300 F F NA Agree 153.09 0.00 0.00 0.00 0.00 0.00 NONE POLYA 0.89485 0.054379 0.049133 0.001638 94.42 3.23 1.96 73 LP2 (2026-09-30) PASS NonPolyA_perM 73 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM4447301 F F NA Agree 153.36 0.00 0.00 0.00 0.00 0.00 NONE POLYA 0.870764 0.074885 0.05102 0.003331 94.02 3.60 1.91 144 LP2 (2026-09-30) PASS NonPolyA_perM 144 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM4447302 F F NA Agree 182.77 0.00 0.00 0.00 0.00 0.00 NONE POLYA 0.828551 0.10219 0.062398 0.006862 93.49 4.15 1.88 167 LP2 (2026-09-30) PASS NonPolyA_perM 167 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM4447303 F F NA Agree 160.46 0.00 0.00 0.00 0.00 0.00 NONE POLYA 0.821511 0.109258 0.064317 0.004913 93.53 3.92 2.03 147 LP2 (2026-09-30) PASS NonPolyA_perM 147 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM4447304 F F NA Agree 260.69 0.00 0.00 0.00 0.00 0.00 NONE POLYA 0.846395 0.09719 0.055014 0.001401 94.27 3.29 1.98 136 LP2 (2026-09-30) PASS NonPolyA_perM 136 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM4447305 F F NA Agree 181.81 0.00 0.00 0.00 0.00 0.00 NONE POLYA 0.81931 0.113967 0.061213 0.00551 93.23 4.11 2.10 169 LP2 (2026-09-30) PASS NonPolyA_perM 169 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM4447306 F F NA Agree 167.49 0.00 0.00 0.00 0.00 0.00 NONE POLYA 0.869102 0.079667 0.049222 0.002009 94.99 3.18 1.44 102 LP2 (2026-09-30) PASS NonPolyA_perM 102 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM4447307 F F NA Agree 149.59 0.00 0.00 0.00 0.00 0.00 NONE POLYA 0.865254 0.079066 0.053177 0.002504 94.17 3.44 1.96 144 LP2 (2026-09-30) PASS NonPolyA_perM 144 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM4447308 F F NA Agree 131.79 0.00 0.00 0.00 0.00 0.00 NONE POLYA 0.883219 0.068558 0.045766 0.002456 94.33 3.41 1.90 117 LP2 (2026-09-30) PASS NonPolyA_perM 117 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM4447309 F F NA Agree 83.69 0.00 0.00 0.00 0.00 0.00 NONE POLYA 0.893525 0.057415 0.047306 0.001754 94.32 3.37 1.93 89 LP2 (2026-09-30) PASS NonPolyA_perM 89 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM4447310 F F NA Agree 175.49 0.00 0.00 0.00 0.00 0.00 NONE POLYA 0.854894 0.087304 0.055111 0.002691 94.40 3.32 1.85 132 LP2 (2026-09-30) PASS NonPolyA_perM 132 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM4447311 F F NA Agree 91.91 0.00 0.00 0.00 0.00 0.00 NONE POLYA 0.896209 0.050534 0.051357 0.001901 94.79 3.34 1.48 62 LP2 (2026-09-30) PASS NonPolyA_perM 62 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM4447312 F F NA Agree 51.76 0.00 0.00 0.00 0.00 0.00 NONE POLYA 0.91406 0.037808 0.046998 0.001135 94.11 3.61 1.93 59 LP2 (2026-09-30) PASS NonPolyA_perM 59 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM4447313 F F NA Agree 72.54 0.00 0.00 0.00 0.00 0.00 NONE POLYA 0.914459 0.036406 0.045991 0.003144 94.02 3.98 1.60 44 LP2 (2026-09-30) PASS NonPolyA_perM 44 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM4447314 F F NA Agree 181.02 0.00 0.00 0.00 0.00 0.00 NONE POLYA 0.887691 0.065476 0.044932 0.001901 94.00 3.42 2.10 82 LP2 (2026-09-30) PASS NonPolyA_perM 82 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM4447315 F F NA Agree 70.24 0.00 0.00 0.00 0.00 0.00 NONE POLYA 0.917673 0.035905 0.045087 0.001335 94.50 3.06 2.07 52 LP2 (2026-09-30) PASS NonPolyA_perM 52 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM4447316 F F NA Agree 85.08 0.00 0.00 0.00 0.00 0.00 NONE POLYA 0.909145 0.043753 0.045964 0.001139 94.12 3.34 2.20 69 LP2 (2026-09-30) PASS NonPolyA_perM 69 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does