Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-20) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM4437410	M	M	NA	Agree	0.02	4.23	0.00	13.53	16.55	77.07	NONE	POLYA	0.842018	0.10305	0.048983	0.005949	88.57	5.70	5.28	174	LP2 (2026-09-30)	PASS	NonPolyA_perM 174 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4437411	F	F	NA	Agree	31.29	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.86346	0.088805	0.045854	0.001881	87.20	5.70	6.66	147	LP2 (2026-09-30)	PASS	NonPolyA_perM 147 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4437412	M	M	NA	Agree	0.03	3.75	0.00	13.83	16.80	90.44	NONE	POLYA	0.847178	0.103002	0.048412	0.001408	88.32	5.49	5.76	158	LP2 (2026-09-30)	PASS	NonPolyA_perM 158 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4437413	M	M	NA	Agree	0.08	3.46	0.00	13.07	14.89	81.74	NONE	POLYA	0.84908	0.101754	0.048004	0.001163	88.78	5.04	5.76	165	LP2 (2026-09-30)	PASS	NonPolyA_perM 165 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4437414	F	F	NA	Agree	52.18	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.85193	0.098178	0.048654	0.001237	88.20	5.26	6.09	156	LP2 (2026-09-30)	PASS	NonPolyA_perM 156 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4437415	M	M	NA	Agree	0.07	2.59	0.00	10.34	13.26	77.21	NONE	POLYA	0.853574	0.095386	0.050019	0.001021	89.09	5.31	5.16	158	LP2 (2026-09-30)	PASS	NonPolyA_perM 158 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does