Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-20) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM4318357	M	M	NA	Agree	0.01	6.23	0.00	52.31	19.21	63.06	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.886471	0.068855	0.042698	0.001976	96.07	2.13	1.54	74	LP2 (2026-09-30)	PASS	NonPolyA_perM 74 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4318358	M	M	NA	Agree	0.01	6.87	0.00	58.72	19.16	68.14	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.875969	0.073043	0.04422	0.006768	95.62	2.61	1.51	78	LP2 (2026-09-30)	PASS	NonPolyA_perM 78 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4318359	M	M	NA	Agree	0.00	5.81	0.00	56.98	18.64	66.95	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.884251	0.069941	0.04349	0.002317	96.08	2.17	1.48	73	LP2 (2026-09-30)	PASS	NonPolyA_perM 73 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4318360	M	M	NA	Agree	0.00	6.88	0.00	54.49	18.94	64.11	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.886484	0.067723	0.043582	0.002211	96.10	2.17	1.48	73	LP2 (2026-09-30)	PASS	NonPolyA_perM 73 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4318361	M	M	NA	Agree	0.00	6.43	0.00	55.87	20.11	70.24	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.885439	0.06903	0.043577	0.001954	96.05	2.18	1.51	75	LP2 (2026-09-30)	PASS	NonPolyA_perM 75 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4318362	M	M	NA	Agree	0.01	5.40	0.00	53.48	18.05	62.76	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.886961	0.06831	0.042582	0.002147	96.00	2.16	1.57	68	LP2 (2026-09-30)	PASS	NonPolyA_perM 68 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does