Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-21) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM4143741	not_specified	F	NA	Conflict	214.37	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.806921	0.154142	0.038777	0.000159	95.08	1.92	2.78	170	LP2 (2026-09-30)	PASS	NonPolyA_perM 170 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4143742	not_specified	F	NA	Conflict	229.69	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.829569	0.138855	0.031409	0.000168	95.50	1.87	2.44	142	LP2 (2026-09-30)	PASS	NonPolyA_perM 142 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4143743	not_specified	F	NA	Conflict	232.53	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.803842	0.161051	0.03493	0.000177	95.21	1.81	2.77	193	LP2 (2026-09-30)	PASS	NonPolyA_perM 193 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4143744	not_specified	M	NA	Conflict	0.00	11.56	0.00	66.37	13.45	68.22	NONE	POLYA	0.851838	0.115223	0.032658	0.000281	95.33	1.74	2.71	124	LP2 (2026-09-30)	PASS	NonPolyA_perM 124 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4143745	not_specified	M	NA	Conflict	0.01	7.53	0.00	57.42	10.61	67.06	NONE	POLYA	0.856771	0.115345	0.027547	0.000338	95.15	1.81	2.86	137	LP2 (2026-09-30)	PASS	NonPolyA_perM 137 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4143746	not_specified	M	NA	Conflict	0.05	10.10	0.00	63.64	16.87	73.62	NONE	POLYA	0.848125	0.120811	0.030769	0.000295	95.24	1.76	2.81	146	LP2 (2026-09-30)	PASS	NonPolyA_perM 146 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4143747	not_specified	M	NA	Conflict	0.01	4.12	0.00	29.00	9.71	56.63	NONE	POLYA	0.893464	0.082315	0.023904	0.000317	95.63	2.00	2.18	102	LP2 (2026-09-30)	PASS	NonPolyA_perM 102 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4143748	not_specified	F	NA	Conflict	127.52	0.04	0.00	0.14	0.00	0.47	NONE	POLYA	0.885915	0.086625	0.027185	0.000275	95.57	1.88	2.35	121	LP2 (2026-09-30)	PASS	NonPolyA_perM 121 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4143749	not_specified	M	NA	Conflict	1.20	5.98	0.00	36.61	9.66	57.62	NONE	POLYA	0.865317	0.104958	0.029363	0.000362	95.04	1.93	2.81	158	LP2 (2026-09-30)	PASS	NonPolyA_perM 158 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4143750	not_specified	M	NA	Conflict	1.42	5.54	0.00	33.78	8.46	60.08	NONE	POLYA	0.884967	0.081527	0.032144	0.001362	94.31	2.13	3.34	133	LP2 (2026-09-30)	PASS	NonPolyA_perM 133 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4143751	not_specified	F	NA	Conflict	165.71	0.05	0.00	0.86	0.12	0.48	NONE	POLYA	0.862404	0.108342	0.028722	0.000532	94.97	2.05	2.78	147	LP2 (2026-09-30)	PASS	NonPolyA_perM 147 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4143752	not_specified	F	NA	Conflict	203.41	0.00	0.00	0.50	0.12	0.17	NONE	POLYA	0.867278	0.108052	0.024318	0.000352	94.77	2.08	2.94	122	LP2 (2026-09-30)	PASS	NonPolyA_perM 122 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does