Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-22) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM4061770	M	M	NA	Agree	0.01	8.79	0.00	52.10	21.94	93.80	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.843867	0.096184	0.045488	0.014461	80.74	3.20	15.84	93	LP2 (2026-09-30)	PASS	NonPolyA_perM 93 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4061771	M	M	NA	Agree	0.00	10.27	0.00	46.34	24.29	102.93	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.847682	0.101184	0.04593	0.005204	81.50	2.24	16.06	91	LP2 (2026-09-30)	PASS	NonPolyA_perM 91 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4061772	M	M	NA	Agree	0.00	6.12	0.00	33.41	20.69	63.61	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.869427	0.084123	0.042159	0.004291	72.35	2.06	25.36	83	LP2 (2026-09-30)	PASS	NonPolyA_perM 83 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4061773	M	M	NA	Agree	0.02	7.76	0.00	35.41	19.85	84.35	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.855985	0.08457	0.04304	0.016404	94.54	3.39	1.81	86	LP2 (2026-09-30)	PASS	NonPolyA_perM 86 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4061774	M	M	NA	Agree	0.00	5.37	0.00	33.88	21.52	58.87	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.869554	0.081291	0.040749	0.008406	74.73	2.55	22.48	85	LP2 (2026-09-30)	PASS	NonPolyA_perM 85 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4061775	M	M	NA	Agree	0.01	5.59	0.00	29.96	16.13	86.38	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.874865	0.060716	0.040119	0.0243	92.87	4.39	2.48	85	LP2 (2026-09-30)	PASS	NonPolyA_perM 85 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4061776	M	M	NA	Agree	0.00	8.23	0.00	39.19	20.35	88.93	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.856389	0.092568	0.044393	0.006649	82.50	2.46	14.84	86	LP2 (2026-09-30)	PASS	NonPolyA_perM 86 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4061777	M	M	NA	Agree	0.01	7.40	0.00	36.24	19.21	82.22	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.859887	0.073424	0.042323	0.024366	94.06	4.21	1.40	95	LP2 (2026-09-30)	PASS	NonPolyA_perM 95 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4061778	M	M	NA	Agree	0.01	7.58	0.00	40.94	20.49	88.58	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.862579	0.083869	0.044747	0.008805	80.55	2.62	16.61	93	LP2 (2026-09-30)	PASS	NonPolyA_perM 93 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4061779	M	M	NA	Agree	0.00	5.64	0.00	26.21	14.74	83.12	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.854589	0.060904	0.038708	0.0458	90.93	6.39	2.42	90	LP2 (2026-09-30)	PASS	NonPolyA_perM 90 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4061780	M	M	NA	Agree	0.00	6.11	0.00	29.44	16.17	91.71	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.87666	0.05873	0.040673	0.023938	91.75	4.23	3.07	73	LP2 (2026-09-30)	PASS	NonPolyA_perM 73 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4061781	M	M	NA	Agree	0.02	6.19	0.00	29.73	18.31	54.05	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.868332	0.080501	0.042294	0.008873	60.49	2.39	36.86	97	LP2 (2026-09-30)	PASS	NonPolyA_perM 97 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4061782	M	M	NA	Agree	0.00	5.72	0.00	29.53	16.06	72.86	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.850151	0.061011	0.040026	0.048812	90.84	6.63	2.27	106	LP2 (2026-09-30)	PASS	NonPolyA_perM 106 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4061783	M	M	NA	Agree	0.01	10.55	0.00	50.66	21.62	96.70	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.841356	0.100088	0.046028	0.012527	80.60	2.92	16.26	120	LP2 (2026-09-30)	PASS	NonPolyA_perM 120 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4061784	M	M	NA	Agree	0.01	6.19	0.00	38.97	16.28	87.02	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.871775	0.064642	0.03958	0.024002	93.75	4.33	1.66	86	LP2 (2026-09-30)	PASS	NonPolyA_perM 86 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4061785	M	M	NA	Agree	0.01	6.90	0.00	33.24	18.23	91.32	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.872429	0.065286	0.041688	0.020597	94.42	4.03	1.26	91	LP2 (2026-09-30)	PASS	NonPolyA_perM 91 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does