Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-21) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM3926629	F	F	NA	Agree	150.09	0.00	0.00	0.09	0.00	0.00	NONE	POLYA	0.885481	0.078101	0.03468	0.001738	96.55	1.53	1.78	94	LP2 (2026-09-30)	PASS	NonPolyA_perM 94 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM3926630	F	F	NA	Agree	188.72	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.873058	0.090145	0.035625	0.001172	96.11	1.37	2.39	80	LP2 (2026-09-30)	PASS	NonPolyA_perM 80 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM3926631	F	F	NA	Agree	121.33	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.907003	0.054813	0.036589	0.001595	95.88	1.73	2.28	59	LP2 (2026-09-30)	PASS	NonPolyA_perM 59 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM3926632	F	F	NA	Agree	117.17	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.896094	0.066591	0.036169	0.001147	96.34	1.59	1.93	68	LP2 (2026-09-30)	PASS	NonPolyA_perM 68 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM3926633	F	F	NA	Agree	118.09	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.898267	0.067337	0.032551	0.001845	95.93	1.73	2.20	77	LP2 (2026-09-30)	PASS	NonPolyA_perM 77 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM3926634	F	F	NA	Agree	105.54	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.897696	0.065286	0.03273	0.004288	95.04	2.24	2.57	73	LP2 (2026-09-30)	PASS	NonPolyA_perM 73 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM3926635	F	F	NA	Agree	142.36	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.886416	0.078057	0.034351	0.001177	95.82	1.92	2.09	89	LP2 (2026-09-30)	PASS	NonPolyA_perM 89 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM3926636	F	F	NA	Agree	117.38	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.893895	0.069233	0.035259	0.001612	95.65	1.86	2.31	67	LP2 (2026-09-30)	PASS	NonPolyA_perM 67 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM3926637	F	F	NA	Agree	156.89	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.874311	0.087521	0.034206	0.003962	95.38	1.84	2.61	104	LP2 (2026-09-30)	PASS	NonPolyA_perM 104 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM3926638	F	F	NA	Agree	151.83	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.887587	0.076266	0.034384	0.001763	95.62	1.72	2.50	100	LP2 (2026-09-30)	PASS	NonPolyA_perM 100 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM3926639	F	F	NA	Agree	185.45	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.881754	0.083913	0.033694	0.000639	96.03	1.67	2.14	102	LP2 (2026-09-30)	PASS	NonPolyA_perM 102 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM3926640	F	F	NA	Agree	150.77	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.891326	0.073086	0.034418	0.00117	95.91	1.58	2.35	83	LP2 (2026-09-30)	PASS	NonPolyA_perM 83 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does