Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-16) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO). SampleID InputSex ComputedSex XYRatio Agreement Xist Uty Sry Ddx3y Kdm5d Eif2s3y ComputedStrand ComputedLibraryPrep PctMRNA PctIntronic PctIntergenic PctRibosomal UniqPct MultiPct TooShortPct NonPolyA_perM LibraryPrepRule LibraryPrepQC LibraryPrepReason GSM3575368 M M NA Agree 0.00 10.73 0.00 55.41 27.76 65.34 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.865656 0.087496 0.04354 0.003307 89.97 3.70 6.13 153 LP2 (2026-09-30) PASS NonPolyA_perM 153 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM3575369 M M NA Agree 0.25 5.67 0.00 35.01 18.24 66.42 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.876959 0.072234 0.048321 0.002486 90.05 2.28 7.49 80 LP2 (2026-09-30) PASS NonPolyA_perM 80 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM3575370 M M NA Agree 0.03 7.03 1.28 45.80 30.00 62.37 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.838086 0.113804 0.042845 0.005264 87.35 3.45 8.81 116 LP2 (2026-09-30) PASS NonPolyA_perM 116 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM3575371 M M NA Agree 0.00 4.84 0.00 32.28 9.78 49.31 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.907143 0.063429 0.023811 0.005616 89.47 2.78 7.59 73 LP2 (2026-09-30) PASS NonPolyA_perM 73 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM3575372 M M NA Agree 0.01 2.39 0.00 28.89 9.07 35.46 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.908378 0.062311 0.022358 0.006953 94.22 3.56 2.03 48 LP2 (2026-09-30) PASS NonPolyA_perM 48 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM3575373 M M NA Agree 0.01 9.46 0.00 52.65 34.81 65.67 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.831618 0.120469 0.044315 0.003598 92.73 3.63 3.19 123 LP2 (2026-09-30) PASS NonPolyA_perM 123 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM3575374 M M NA Agree 0.00 3.36 0.00 32.55 7.38 35.36 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.945386 0.032603 0.01936 0.002652 93.71 2.67 3.48 22 LP2 (2026-09-30) PASS NonPolyA_perM 22 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM3575375 M M NA Agree 0.22 5.00 0.00 29.18 14.83 56.39 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.888927 0.069905 0.033619 0.007549 89.71 3.47 6.00 100 LP2 (2026-09-30) PASS NonPolyA_perM 100 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM3575376 M M NA Agree 0.06 8.45 0.00 40.74 21.37 58.78 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.818001 0.137168 0.03791 0.00692 92.72 5.30 1.80 230 LP2 (2026-09-30) PASS NonPolyA_perM 230 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM3575377 M M NA Agree 0.01 5.42 0.00 26.34 12.47 42.66 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.903732 0.062442 0.026163 0.007662 89.60 3.18 6.98 87 LP2 (2026-09-30) PASS NonPolyA_perM 87 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM3575378 F F NA Agree 146.04 0.00 0.00 0.00 0.00 0.00 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.861522 0.0914 0.039823 0.007256 89.49 3.10 7.05 108 LP2 (2026-09-30) PASS NonPolyA_perM 108 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM3575379 M M NA Agree 0.04 9.41 0.00 56.65 25.13 70.98 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.870227 0.083447 0.041507 0.004819 88.73 3.89 7.14 140 LP2 (2026-09-30) PASS NonPolyA_perM 140 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM3575380 M M NA Agree 0.04 8.18 0.00 51.29 20.58 81.24 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.833479 0.111674 0.049546 0.005301 95.42 2.05 2.34 94 LP2 (2026-09-30) PASS NonPolyA_perM 94 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM3575381 M M NA Agree 0.03 6.99 0.00 48.10 27.26 70.25 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.856796 0.09929 0.041854 0.00206 89.46 3.61 6.44 136 LP2 (2026-09-30) PASS NonPolyA_perM 136 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM3575382 M M NA Agree 0.53 5.08 0.00 27.16 8.80 51.12 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.912115 0.060372 0.024907 0.002606 85.78 2.56 11.52 59 LP2 (2026-09-30) PASS NonPolyA_perM 59 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM3575383 M M NA Agree 0.00 2.68 0.00 28.13 8.32 36.82 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.909414 0.061323 0.022954 0.006309 88.98 4.15 6.67 57 LP2 (2026-09-30) PASS NonPolyA_perM 57 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM3575384 M M NA Agree 0.00 6.55 0.00 35.01 24.68 71.82 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.845923 0.108284 0.04071 0.005082 87.88 4.08 7.64 159 LP2 (2026-09-30) PASS NonPolyA_perM 159 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM3575385 M M NA Agree 0.01 3.23 0.00 33.60 6.38 35.93 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.946872 0.031713 0.019317 0.002099 92.27 2.75 4.88 33 LP2 (2026-09-30) PASS NonPolyA_perM 33 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM3575386 M M NA Agree 0.00 9.29 0.00 45.68 25.60 62.55 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.827789 0.122738 0.044993 0.00448 93.04 2.04 4.66 208 LP2 (2026-09-30) PASS NonPolyA_perM 208 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM3575387 M M NA Agree 0.03 5.08 0.00 27.42 12.55 60.69 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.880982 0.068266 0.032845 0.017907 88.15 3.43 7.79 97 LP2 (2026-09-30) PASS NonPolyA_perM 97 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM3575388 M M NA Agree 0.02 4.54 0.00 23.68 13.60 49.91 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.877757 0.078645 0.040072 0.003526 81.95 8.92 8.65 221 LP2 (2026-09-30) PASS NonPolyA_perM 221 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM3575389 M M NA Agree 0.00 8.70 0.00 45.02 22.92 63.29 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.875663 0.084875 0.034777 0.004686 92.93 2.01 4.89 99 LP2 (2026-09-30) PASS NonPolyA_perM 99 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM3575390 F F NA Agree 146.68 0.02 0.00 0.00 0.01 0.05 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.87021 0.081137 0.043303 0.005349 89.69 4.07 6.03 148 LP2 (2026-09-30) PASS NonPolyA_perM 148 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM3575391 F F NA Agree 216.63 0.00 0.00 0.05 0.02 0.01 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.834459 0.109677 0.04816 0.007704 93.33 2.21 4.28 104 LP2 (2026-09-30) PASS NonPolyA_perM 104 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM3575392 F F NA Agree 84.82 0.04 0.00 0.46 0.12 0.55 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.915568 0.059463 0.022548 0.002422 89.22 2.40 8.24 54 LP2 (2026-09-30) PASS NonPolyA_perM 54 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM3575393 F F NA Agree 109.31 0.00 0.00 0.00 0.01 0.02 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.910797 0.062316 0.021533 0.005354 87.11 3.45 9.25 51 LP2 (2026-09-30) PASS NonPolyA_perM 51 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM3575394 F F NA Agree 160.86 0.00 0.00 0.00 0.00 0.00 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.846388 0.110527 0.041042 0.002042 92.37 3.79 3.43 139 LP2 (2026-09-30) PASS NonPolyA_perM 139 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM3575395 F F NA Agree 37.61 0.00 0.00 0.00 0.00 0.00 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.936111 0.036303 0.020004 0.007582 96.06 2.23 1.58 35 LP2 (2026-09-30) PASS NonPolyA_perM 35 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM3575396 F F NA Agree 178.17 0.00 0.00 0.00 0.00 0.00 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.821431 0.130764 0.044074 0.003731 95.55 2.14 1.98 254 LP2 (2026-09-30) PASS NonPolyA_perM 254 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM3575397 F F NA Agree 108.44 0.00 0.00 0.00 0.00 0.03 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.878614 0.079742 0.036614 0.005029 89.91 3.18 6.65 115 LP2 (2026-09-30) PASS NonPolyA_perM 115 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM3575398 F F NA Agree 135.02 0.00 0.00 0.00 0.00 0.00 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.816544 0.137055 0.039408 0.006992 91.69 4.36 3.78 257 LP2 (2026-09-30) PASS NonPolyA_perM 257 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM3575399 F F NA Agree 87.75 0.00 0.00 0.00 0.00 0.00 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.903424 0.057362 0.034863 0.004351 92.77 2.99 4.01 81 LP2 (2026-09-30) PASS NonPolyA_perM 81 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM3575400 M M NA Agree 0.00 7.83 0.00 49.47 21.11 65.46 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.85818 0.088651 0.045571 0.007597 94.25 3.40 2.16 142 LP2 (2026-09-30) PASS NonPolyA_perM 142 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM3575401 M M NA Agree 0.06 7.87 0.00 43.31 17.84 66.86 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.846235 0.101836 0.047601 0.004327 90.07 2.48 7.25 96 LP2 (2026-09-30) PASS NonPolyA_perM 96 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM3575402 M M NA Agree 0.07 8.58 0.95 48.61 28.15 65.36 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.840724 0.113229 0.04088 0.005167 90.10 2.96 6.51 120 LP2 (2026-09-30) PASS NonPolyA_perM 120 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM3575403 M M NA Agree 0.00 4.52 0.00 26.85 8.23 49.51 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.91616 0.055153 0.0215 0.007187 95.30 2.46 2.13 61 LP2 (2026-09-30) PASS NonPolyA_perM 61 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM3575404 M M NA Agree 0.01 2.37 0.00 25.70 8.14 28.54 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.914498 0.058706 0.024091 0.002706 85.35 4.05 10.39 60 LP2 (2026-09-30) PASS NonPolyA_perM 60 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM3575405 M M NA Agree 0.00 9.00 0.00 57.62 33.61 57.66 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.839845 0.115831 0.04164 0.002684 92.22 2.34 5.19 108 LP2 (2026-09-30) PASS NonPolyA_perM 108 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM3575406 M M NA Agree 0.00 4.10 0.00 43.07 8.35 46.54 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.938079 0.038412 0.019814 0.003695 94.98 1.94 3.00 36 LP2 (2026-09-30) PASS NonPolyA_perM 36 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM3575407 M M NA Agree 0.00 4.99 0.00 22.73 13.60 56.23 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.839209 0.083533 0.044509 0.032749 88.63 6.53 4.51 195 LP2 (2026-09-30) PASS NonPolyA_perM 195 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM3575408 M M NA Agree 0.08 8.89 0.00 44.20 21.85 52.99 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.824682 0.13061 0.039909 0.0048 77.05 6.68 15.86 245 LP2 (2026-09-30) PASS NonPolyA_perM 245 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM3575409 M M NA Agree 0.00 5.48 0.00 28.62 11.98 49.33 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.905457 0.065431 0.027047 0.002065 92.23 2.72 4.81 73 LP2 (2026-09-30) PASS NonPolyA_perM 73 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM3575410 F F NA Agree 161.51 0.00 0.00 0.00 0.00 0.00 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.873519 0.080681 0.040271 0.005529 90.35 3.10 6.40 143 LP2 (2026-09-30) PASS NonPolyA_perM 143 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM3575411 F F NA Agree 185.70 0.00 0.00 0.00 0.00 0.00 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.849011 0.098446 0.046188 0.006354 89.51 2.59 7.69 85 LP2 (2026-09-30) PASS NonPolyA_perM 85 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM3575412 F F NA Agree 134.24 0.01 0.00 0.05 0.01 0.23 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.864391 0.091929 0.040584 0.003096 89.86 3.75 5.85 135 LP2 (2026-09-30) PASS NonPolyA_perM 135 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM3575413 F F NA Agree 110.35 0.00 0.00 0.00 0.01 0.06 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.905458 0.067487 0.024398 0.002657 86.55 2.47 10.83 66 LP2 (2026-09-30) PASS NonPolyA_perM 66 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM3575414 F F NA Agree 110.47 0.00 0.00 0.00 0.00 0.00 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.910452 0.063237 0.019823 0.006487 92.98 2.95 3.93 70 LP2 (2026-09-30) PASS NonPolyA_perM 70 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM3575415 F F NA Agree 109.26 0.00 0.00 0.01 0.00 0.01 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.846009 0.108276 0.038818 0.006897 91.19 2.90 5.64 125 LP2 (2026-09-30) PASS NonPolyA_perM 125 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM3575416 F F NA Agree 23.95 0.00 0.00 0.00 0.00 0.00 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.94436 0.032826 0.019998 0.002815 89.71 2.73 7.43 26 LP2 (2026-09-30) PASS NonPolyA_perM 26 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM3575417 F F NA Agree 183.38 0.00 0.00 0.00 0.00 0.00 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.834805 0.114681 0.048723 0.001791 72.79 2.55 24.36 237 LP2 (2026-09-30) PASS NonPolyA_perM 237 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM3575418 F F NA Agree 101.24 0.00 0.00 0.00 0.02 0.04 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.887954 0.073407 0.035477 0.003162 90.07 3.23 6.41 107 LP2 (2026-09-30) PASS NonPolyA_perM 107 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM3575419 F F NA Agree 126.30 0.00 0.00 0.07 0.02 0.05 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.815075 0.135276 0.040308 0.009341 91.07 5.53 3.10 267 LP2 (2026-09-30) PASS NonPolyA_perM 267 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM3575420 F F NA Agree 111.36 0.00 0.00 0.00 0.00 0.00 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.88463 0.074518 0.035754 0.005098 89.49 2.98 7.32 90 LP2 (2026-09-30) PASS NonPolyA_perM 90 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM3575421 F F NA Agree 157.61 0.00 0.00 0.03 0.03 0.11 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.87918 0.073056 0.044281 0.003483 83.30 4.19 12.29 153 LP2 (2026-09-30) PASS NonPolyA_perM 153 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM3575422 F F NA Agree 200.98 0.00 0.00 0.00 0.00 0.00 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.842255 0.111306 0.042783 0.003656 91.27 3.42 4.87 137 LP2 (2026-09-30) PASS NonPolyA_perM 137 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM3575423 F F NA Agree 43.62 0.00 0.00 0.01 0.00 0.05 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.932064 0.040204 0.020825 0.006906 95.27 2.40 2.24 34 LP2 (2026-09-30) PASS NonPolyA_perM 34 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM3575424 F F NA Agree 126.99 0.00 0.00 0.00 0.00 0.02 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.906508 0.067369 0.023696 0.002427 88.67 3.64 7.45 65 LP2 (2026-09-30) PASS NonPolyA_perM 65 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM3575425 F F NA Agree 49.60 0.00 0.00 0.01 0.01 0.00 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.885619 0.064195 0.045104 0.005082 85.42 4.87 9.36 142 LP2 (2026-09-30) PASS NonPolyA_perM 142 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM3575426 F F NA Agree 25.00 0.02 0.00 0.02 0.02 0.10 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.943815 0.033029 0.020065 0.003091 84.74 2.62 12.55 21 LP2 (2026-09-30) PASS NonPolyA_perM 21 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM3575427 F F NA Agree 151.08 0.00 0.00 0.00 0.00 0.00 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.834121 0.120686 0.041199 0.003993 93.52 2.74 3.39 253 LP2 (2026-09-30) PASS NonPolyA_perM 253 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM3575428 F F NA Agree 78.75 0.00 0.00 0.05 0.01 0.31 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.902633 0.059807 0.033395 0.004164 94.81 2.31 2.51 79 LP2 (2026-09-30) PASS NonPolyA_perM 79 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM3575429 F F NA Agree 174.09 0.00 0.00 0.00 0.00 0.00 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.805729 0.147895 0.041799 0.004578 83.99 5.19 10.51 299 LP2 (2026-09-30) PASS NonPolyA_perM 299 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM3575430 F F NA Agree 105.36 0.00 0.00 0.00 0.00 0.00 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.891701 0.072509 0.031131 0.004659 89.69 2.07 8.05 83 LP2 (2026-09-30) PASS NonPolyA_perM 83 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM3575431 F F NA Agree 113.72 0.00 0.00 0.02 0.01 0.00 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.881264 0.075562 0.03475 0.008424 95.21 2.66 1.98 85 LP2 (2026-09-30) PASS NonPolyA_perM 85 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM3575432 F F NA Agree 129.33 0.00 0.00 0.02 0.02 0.05 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.870582 0.091056 0.035663 0.002699 89.16 2.67 7.95 102 LP2 (2026-09-30) PASS NonPolyA_perM 102 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM3575433 F F NA Agree 214.72 0.00 0.00 0.00 0.00 0.00 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.829879 0.115621 0.050966 0.003534 92.71 3.81 3.08 158 LP2 (2026-09-30) PASS NonPolyA_perM 158 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM3575434 M M NA Agree 0.18 7.28 0.00 42.87 19.83 71.74 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.874147 0.084907 0.035822 0.005124 93.73 2.47 3.67 107 LP2 (2026-09-30) PASS NonPolyA_perM 107 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM3575435 M M NA Agree 0.00 6.11 0.00 43.58 20.56 65.71 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.872291 0.084223 0.035755 0.007731 91.89 3.23 4.67 88 LP2 (2026-09-30) PASS NonPolyA_perM 88 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM3575436 M M NA Agree 0.27 6.02 0.00 27.72 19.86 63.50 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.883005 0.076148 0.034645 0.006202 91.14 2.95 5.68 91 LP2 (2026-09-30) PASS NonPolyA_perM 91 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM3575437 M M NA Agree 0.42 2.69 0.00 5.56 1.73 32.73 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.787302 0.091824 0.055496 0.065378 88.70 8.19 2.18 51 LP2 (2026-09-30) PASS NonPolyA_perM 51 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM3575438 M M NA Agree 0.39 2.62 0.00 5.41 1.57 30.41 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.789697 0.088203 0.054611 0.067489 88.04 8.34 2.71 55 LP2 (2026-09-30) PASS NonPolyA_perM 55 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM3575439 M M NA Agree 0.38 2.48 0.00 5.22 2.12 29.98 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.784103 0.088707 0.055613 0.071577 83.81 9.15 6.14 46 LP2 (2026-09-30) PASS NonPolyA_perM 46 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM3575440 M M NA Agree 0.40 2.49 0.00 5.01 1.35 30.55 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.816109 0.089312 0.056994 0.037584 90.15 6.29 2.57 44 LP2 (2026-09-30) PASS NonPolyA_perM 44 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM3575441 M M NA Agree 0.41 2.37 0.00 4.91 1.71 27.21 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.797477 0.090304 0.058245 0.053974 88.75 7.91 2.31 61 LP2 (2026-09-30) PASS NonPolyA_perM 61 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM3575442 M M NA Agree 0.34 2.91 0.00 5.11 1.94 31.87 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.785205 0.089255 0.056371 0.069169 81.49 8.64 8.99 54 LP2 (2026-09-30) PASS NonPolyA_perM 54 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM3575443 M M NA Agree 0.47 2.56 0.00 5.05 1.99 30.37 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.789365 0.090034 0.056398 0.064203 79.95 8.10 11.04 49 LP2 (2026-09-30) PASS NonPolyA_perM 49 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does