Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-21) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM3562395	M	M	NA	Agree	0.11	1.40	0.00	12.25	4.65	31.39	NONE	POLYA	0.896109	0.061419	0.028418	0.014054	92.01	3.75	3.67	69	LP2 (2026-09-30)	PASS	NonPolyA_perM 69 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM3562396	M	M	NA	Agree	0.07	1.53	0.00	16.70	6.31	31.12	NONE	POLYA	0.902019	0.067465	0.025224	0.005292	91.95	3.13	4.39	58	LP2 (2026-09-30)	PASS	NonPolyA_perM 58 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM3562397	M	M	NA	Agree	0.08	1.37	0.00	17.07	4.44	30.94	NONE	POLYA	0.887576	0.074028	0.028975	0.009421	91.05	3.37	4.96	75	LP2 (2026-09-30)	PASS	NonPolyA_perM 75 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM3562398	M	M	NA	Agree	0.02	0.53	0.00	3.19	1.66	10.44	NONE	POLYA	0.93459	0.0297	0.025162	0.010548	90.93	3.02	5.66	42	LP2 (2026-09-30)	PASS	NonPolyA_perM 42 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM3562399	M	M	NA	Agree	0.05	0.91	0.00	6.05	2.62	23.48	NONE	POLYA	0.943447	0.022714	0.028844	0.004996	91.68	2.79	4.93	29	LP2 (2026-09-30)	PASS	NonPolyA_perM 29 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM3562400	M	M	NA	Agree	0.68	1.03	0.00	7.52	2.70	16.66	NONE	POLYA	0.819329	0.122141	0.040306	0.018224	89.04	4.13	6.20	109	LP2 (2026-09-30)	PASS	NonPolyA_perM 109 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM3562401	M	M	NA	Agree	0.03	0.15	0.00	2.50	1.11	9.24	NONE	POLYA	0.957563	0.013048	0.018305	0.011084	92.48	3.02	4.00	19	LP2 (2026-09-30)	PASS	NonPolyA_perM 19 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM3562402	M	M	NA	Agree	0.00	0.56	0.00	3.11	1.58	11.16	NONE	POLYA	0.958785	0.017194	0.021953	0.002068	93.59	2.22	3.81	19	LP2 (2026-09-30)	PASS	NonPolyA_perM 19 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM3562403	M	M	NA	Agree	0.00	0.54	0.00	3.68	1.70	13.63	NONE	POLYA	0.965799	0.011736	0.019706	0.002759	93.22	2.59	3.77	13	LP2 (2026-09-30)	PASS	NonPolyA_perM 13 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does