Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-20) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM3391596	M	M	NA	Agree	0.01	14.61	0.00	89.63	30.67	79.40	NONE	POLYA	0.806846	0.142171	0.050983	0.000001	97.18	0.56	2.23	118	LP2 (2026-09-30)	PASS	NonPolyA_perM 118 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM3391597	M	M	NA	Agree	0.01	15.53	0.00	89.58	30.44	82.38	NONE	POLYA	0.804482	0.142031	0.053487	0.000001	97.00	0.60	2.37	126	LP2 (2026-09-30)	PASS	NonPolyA_perM 126 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM3391598	M	M	NA	Agree	0.02	13.09	0.00	85.69	28.82	79.34	NONE	POLYA	0.802316	0.147122	0.05056	0.000002	97.01	0.60	2.37	109	LP2 (2026-09-30)	PASS	NonPolyA_perM 109 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM3391599	M	M	NA	Agree	0.00	15.11	0.00	88.31	31.14	76.13	NONE	POLYA	0.802927	0.142054	0.055018	0.000001	97.08	0.58	2.31	124	LP2 (2026-09-30)	PASS	NonPolyA_perM 124 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM3391600	M	M	NA	Agree	0.00	14.23	0.00	99.05	32.95	78.83	NONE	POLYA	0.799343	0.144485	0.056171	0.000001	97.13	0.57	2.27	129	LP2 (2026-09-30)	PASS	NonPolyA_perM 129 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM3391601	M	M	NA	Agree	0.01	15.83	0.00	92.41	31.39	79.04	NONE	POLYA	0.798985	0.145213	0.055802	0	97.20	0.59	2.17	112	LP2 (2026-09-30)	PASS	NonPolyA_perM 112 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM3391602	M	M	NA	Agree	0.02	15.53	0.00	91.15	31.76	73.25	NONE	POLYA	0.797943	0.145589	0.056467	0	97.06	0.57	2.33	128	LP2 (2026-09-30)	PASS	NonPolyA_perM 128 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM3391603	M	M	NA	Agree	0.01	14.78	0.00	92.49	29.63	80.56	NONE	POLYA	0.803302	0.141247	0.05545	0	97.02	0.60	2.35	130	LP2 (2026-09-30)	PASS	NonPolyA_perM 130 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM3391604	M	M	NA	Agree	0.01	15.91	0.00	100.21	32.19	76.43	NONE	POLYA	0.791019	0.154248	0.054733	0	97.10	0.58	2.29	128	LP2 (2026-09-30)	PASS	NonPolyA_perM 128 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM3391605	M	M	NA	Agree	0.01	15.64	0.00	101.96	32.98	77.50	NONE	POLYA	0.800729	0.152174	0.047092	0.000005	97.14	0.58	2.25	124	LP2 (2026-09-30)	PASS	NonPolyA_perM 124 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM3391606	M	M	NA	Agree	0.00	11.95	0.00	95.34	28.77	76.92	NONE	POLYA	0.807891	0.143635	0.048466	0.000008	97.21	0.55	2.21	117	LP2 (2026-09-30)	PASS	NonPolyA_perM 117 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM3391607	M	M	NA	Agree	0.01	12.71	0.00	89.01	30.33	76.93	NONE	POLYA	0.801298	0.149899	0.048803	0.000001	97.17	0.58	2.22	113	LP2 (2026-09-30)	PASS	NonPolyA_perM 113 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does