From bernd.koelsch at uni-essen.de Thu May 6 14:54:46 1999 From: bernd.koelsch at uni-essen.de (Bernd Koelsch) Date: Thu, 06 May 1999 13:54:46 -0600 Subject: [rat-forum] New rat marker Message-ID: Hi, Last week I saw at the IOWA-RAT-Project homepage rat marker called (for example) D10Gotxx. My question is: - where can I find a map/intrgrated map of this "Got"-markers - where can I find the sequence of this markers Thanks Bernd ********************************************************* Dr. Bernd Koelsch / e-mail: Bernd.koelsch at uni-essen.de Inst. for Cell Biology (Cancer Research) University of Essen Hufelandstrasse 55 45122 Essen, Germany --------------766A10372248FB4377E4A541 >Content-Type: text/html; charset=us-ascii >Content-Transfer-Encoding: 7bit Hi,

Last week I saw at the IOWA-RAT-Project homepage rat marker called (for example) D10Gotxx.
My question is:
- where can I find a map/intrgrated map of this "Got"-markers
- where can I find the sequence of this markers

Thanks Bernd

*********************************************************
Dr. Bernd Koelsch   /  e-mail:  Bernd.koelsch at uni-essen.de
Inst. for Cell Biology (Cancer Research)
University of Essen
Hufelandstrasse 55
45122 Essen, Germany --------------766A10372248FB4377E4A541-- From jacob at mcw.edu Thu May 6 11:20:51 1999 From: jacob at mcw.edu (Howard Jacob) Date: Thu, 06 May 1999 10:20:51 -0600 Subject: [rat-forum] Re: New rat marker Message-ID: The Got Marker information is in several places depending upon the data y= ou are looking for. In the May issue of Nature Genetics the data are released and several web= sites are listed. We have also placed many of these markers relative to the Whitehead Markers.= These data can be located at http://goliath.ifrc.mcw.edu/LGR and look under the new map rel= eases. Howard Jacob Bernd K=F6lsch wrote: > --------------766A10372248FB4377E4A541 > Content-Type: text/plain; charset=3Dus-ascii; x-mac-type=3D"54455854"; = x-mac-creator=3D"4D4F5353" > Content-Transfer-Encoding: 7bit > > Hi, > > Last week I saw at the IOWA-RAT-Project homepage rat marker called (for > example) D10Gotxx. > My question is: > - where can I find a map/intrgrated map of this "Got"-markers > - where can I find the sequence of this markers > > Thanks Bernd > > ********************************************************* > Dr. Bernd Koelsch / e-mail: Bernd.koelsch at uni-essen.de > Inst. for Cell Biology (Cancer Research) > University of Essen > Hufelandstrasse 55 > 45122 Essen, Germany > > --------------766A10372248FB4377E4A541 > Content-Type: text/html; charset=3Dus-ascii > Content-Transfer-Encoding: 7bit > > > > Hi, >

Last week I saw at the IOWA-RAT-Project homepage rat marker called (= for > example) D10Gotxx. >
My question is: >
- where can I find a map/intrgrated map of this "Got"-markers >
- where can I find the sequence of this markers >

Thanks Bernd >

********************************************************* >
Dr. Bernd Koelsch   /  e-mail:  Bernd.koelsch at u= ni-essen.de >
Inst. for Cell Biology (Cancer Research) >
University of Essen >
Hufelandstrasse 55 >
45122 Essen, Germany > > --------------766A10372248FB4377E4A541-- From nobody at no-reply.mcw.edu Wed May 12 16:42:35 1999 From: nobody at no-reply.mcw.edu (jbm@aretha.jax.org) Date: Wed, 12 May 1999 15:42:35 -0600 Subject: [rat-forum] Experimental Mouse Genetics Course at The Jackson Laboratory Message-ID: COURSE ANNOUNCEMENT: EXPERIMENTAL GENETICS OF THE LABORATORY MOUSE IN CANCER RESEARCH August 21 - September 1, 1999 (Application deadline: JULY 1) Course Organizers: Jackson Laboratory Research Staff FOCUS Topics to be covered include: the genetics of inbred mouse strains; spontaneous/ induced mutants; genetic mapping; physical mapping; candidate gene identification; complex trait analysis; mutagenesis and phenotyping; bioinformatics in genetics and genomics; signaling in growth and development; growth control and malignancy; and mouse models for cancer research. Workshops will include: mouse genome informatics; genetic mapping; laboratory animal husbandry; and tumor histopathology. FACULTY LIST Kenneth Paigen, Ph.D., Director, The Jackson Laboratory Lee Silver, Ph.D., Princeton University Barbara B. Knowles, Ph.D., The Jackson Laboratory Eva Eicher, Ph.D., The Jackson Laboratory John Macauley, Ph.D., The Jackson Laboratory Neal Copeland, Ph.D., National Cancer Institute Muriel Davisson, Ph.D., The Jackson Laboratory John Schimenti, Ph.D., The Jackson Laboratory Garland Allen, Ph.D., Washington University Nancy Jenkins, Ph.D., National Cancer Institute Michael Festing, Ph.D., University of Leicester Joseph Nadeau, Ph.D., Case Western Reserve University Gary Churchill, Ph.D., The Jackson Laboratory Nicholas Schork, Ph.D., The Jackson Laboratory Peter Cooper, Ph.D., National Center for Biotechnology Information Janan Eppig, Ph.D., The Jackson Laboratory Terrie Cunliffe-Beamer, DVM, MS, The Jackson Laboratory John Eppig, Ph.D., The Jackson Laboratory Mary Ann Handel, Ph.D., University of Tennessee Timothy O'brien, Ph.D., The Jackson Laboratory Thomas Gridley, Ph.D., The Jackson Laboratory Frank Ruddle, Ph.D., Yale University Frank Costantini, Ph.D., Columbia University John Sundberg, DVM, Ph.D., The Jackson Laboratory Roderick Bronson, DVM, Tufts University Robert Weinberg, Ph.D., Massachusetts Institute of Technology Peter Demant, Ph.D., The Netherlands Cancer Institute Leonard Shultz, Ph.D., The Jackson Laboratory Raju Kucherlapati, Ph.D., Albert Einstein College of Medicine Wesley Beamer, Ph.D., The Jackson Laboratory Anthony Wynshaw-Boris, Ph.D., U. C. San Diego Gilbert Smith, Ph.D., NCI REGISTRATION FEE The registration fee of $1200.00 includes room and board at the historic Highseas Conference Center. Registration fee waivers are available on a limited basis to highly qualified applicants. ACCOMMODATIONS The course is held in a retreat setting and is limited to 30 students. Both guest faculty members and students reside at Highseas, the Laboratory's conference center/dormitory. APPLICATIONS Enrollment is limited to 30. The course is designed for graduate students and postdoctoral fellows. Application for admission is made by submitting: 1. A typed copy of the following information: Name Mailing Address Telephone Number Fax Number Email Address 2. Your curriculum vitae 3. A letter briefly describing your research and why you wish to attend this course 4. A letter of recommendation from your major professor (for graduate students and postdoctoral fellows). Applications will be reviewed on a competitive selection basis starting in March and will continue until the course is full. For further information, contact Judith Hayward judih at jax.org: Ms. Judith Hayward The Jackson Laboratory 600 Main Street Bar Harbor, Maine 04609 Tel: (207) 288-6326 Fax: (207) 288-6080 Email: judih at jax.org John B. Macauley, Ph.D. Research Scientist Scientific Manager Courses and Meetings The Jackson Laboratory 600 Main St Bar Harbor, Maine 04609-1500 Telephone - (207) 288-6266 Fax - (207) 288-6080 email - jbm at jax.org http://www.jax.org/courses From maehler.michael at mh-hannover.de Sun May 30 10:32:15 1999 From: maehler.michael at mh-hannover.de (Michael Maehler) Date: Sun, 30 May 1999 09:32:15 -0600 Subject: [rat-forum] rat databases Message-ID: Hi, Having found linkage to a genetically mapped marker in the mouse one can search for candidate genes that are located close to this marker by using the Mouse Genome Database. I would like to do the same search in the rat. I am wondering is there any database in the Internet that allows screening for candidate genes in a chromosomal region of interest in the rat? Thank you for your reply. Michael Mahler Michael Mahler, D.V.M. Central Animal Facility and Institute for Laboratory Animal Science Medical School Hannover 30623 Hannover, Germany From tone at mcw.edu Mon May 31 10:12:14 1999 From: tone at mcw.edu (Peter Tonellato) Date: Mon, 31 May 1999 09:12:14 -0600 Subject: [rat-forum] Re: rat databases Message-ID: We have built a comparative map relateing known genetic markers mapped on mouse with those in the human and rat. This tool is not yet fully tested thus is not yet available on-line. However, you can contact me directly for its use in your study. Peter J. Tonellato, Ph.D., Director Informatics Research Center Medical College of Wisconsin 8701 W. Watertown Plank Rd. Milwaukee, WI 53226 tone at mcw.edu 414-456-8871 (Office) 414-456-8870 (Lab) 414-456-6595 (FAX) ----- Original Message ----- Sent: Sunday, May 30, 1999 11:56 PM > Hi, > Having found linkage to a genetically mapped marker in the mouse one can > search for candidate genes that are located close to this marker by using > the Mouse Genome Database. I would like to do the same search in the rat. I > am wondering is there any database in the Internet that allows screening for > candidate genes in a chromosomal region of interest in the rat? > Thank you for your reply. > Michael Mahler > > > Michael Mahler, D.V.M. > Central Animal Facility > and Institute for Laboratory Animal Science > Medical School Hannover > 30623 Hannover, Germany > From nobody at no-reply.mcw.edu Tue May 18 16:29:22 1999 From: nobody at no-reply.mcw.edu (jlaakso@healthtech.com) Date: Tue, 18 May 1999 15:29:22 -0600 Subject: [rat-forum] CHI's ANIMAL MODELS CONFERENCE Message-ID: Hello! Cambridge Healthtech Institute (CHI) is a premier provider of conferences designed to facilitate the exchange of technological advances in the global biomedical industry. We hold over 50 conferences each year that focus on such areas as Genome, Blood Safety, Drug Discovery, Bioprocessing, and Lab Instrumentation. We will be holding a conference that should be of interest to you. The details are as follows: Applications of Genomics to ANIMAL MODELS for Pharmaceutical Studies: Mice and Rats October 20, 1999 Boston, Massachusetts This conference will be held in conjuction with two other meetings. Those details as as follows: CHI's 3rd Annual FUNCTIONAL GENOMICS: Applications of Genomic Technologies to the Understanding of Biological Systems October 18-19, 1999 Boston, Massachusetts CHI's 2nd Annual Implications of HUMAN GENETIC VARIATION: SNP's, Polymorphisms, Diseases & Treatments October 21-22, 1999 Boston, Massachusetts If you would like more details regarding these conferences, please go to our website at: http://www.healthtech.com If this information is not of interest, we appologize for any inconvenience this may have caused. Please email me so that I can remove you from our email list. Best regards, Jennifer Laakso Marketing Coodinator Cambridge Healthtech Institute 1037 Chestnut Street Newton Upper Falls, MA 02464 617-630-1385 direct 617-630-1300 phone 617-630-1325 fax 617-630-1325 fax email: jlaakso at heathtech.com From simont at mcw.edu Tue May 4 18:29:06 1999 From: simont at mcw.edu (Simon Twigger) Date: Tue, 04 May 1999 17:29:06 -0600 Subject: [rat-forum] A High Density Integrated Genetic and Radiation Hybrid Map of the Message-ID: We have prepared files containing all data related to the recently published article: A High Density Integrated Genetic and Radiation Hybrid Map of the Laboratory Rat Steen, etal., Genome Research 1999, May. The data and maps (in gif or pdf format) are available at: http://goliath.ifrc.mcw.edu/LGR/research/rhp/steen_1999.html In addition, our RH placement map server can be found at the same site. These resources are provided through a joint collaboration of The Laboratory for Genetics Research (H.J. Jacob, Ph.D. Director) http://goliath.ifrc.mcw.edu/LGR and The Informatics Research Center (P.J. Tonellato, Director) http://goliath.ifrc.mcw.edu From cilli at ermes.cba.unige.it Mon May 10 17:26:29 1999 From: cilli at ermes.cba.unige.it (Michele Cilli) Date: Mon, 10 May 1999 16:26:29 -0600 Subject: [rat-forum] Superovulation in rats Message-ID: Dear rat lovers, Do you know the dosage of PMSG and hCG to induce superovulation in female rat? Can I use the same for the mouse? Thanks -- Michele Cilli DVM Stabulario IST-CBA Centro Biotecnologie Avanzate L.go Rosanna Benzi, n. 10 16132 Genova Tel.: 0105737274 Fax: 0105737262 E-mail: cilli at ermes.cba.unige.it From nobody at no-reply.mcw.edu Mon May 10 22:46:08 1999 From: nobody at no-reply.mcw.edu (jklohse@facstaff.wisc.edu) Date: Mon, 10 May 1999 21:46:08 -0600 Subject: [rat-forum] Re: Superovulation in rats Message-ID: >Dear rat lovers, > >Do you know the dosage of PMSG and hCG to induce superovulation in >female rat? >Can I use the same for the mouse? > For Fisher344 rats, use females 4-5 weeks old, 80-95g. PMSG will not reprogram the cycles of older animals. Use 15 U of PMSG and 5 U of hCG. In my hands, Sprague-Dawley rats were more difficult to superovulate, and better results were obtained using minipumps dispensing FSH (but this is expensive). Good luck. Email me if you have more questions. I've had reasonable success in making transgenic rats. And I am a rat lover! Dr. Jan K. Lohse Senior Research Specialist Department of Pathobiological Sciences School of Veterinary Medicine 2015 Linden Drive West Madison, WI 53706 (608) 263-9748 From meturner at uakron.edu Tue May 11 14:08:11 1999 From: meturner at uakron.edu (Monte Turner) Date: Tue, 11 May 1999 13:08:11 -0600 Subject: [rat-forum] rat DNA Message-ID: Does anyone know of any source for either animals or DNA from a rat species other than R. norvegicus ? email me for more information or any other questions. Thanks Monte Turner --------------1D00C1CAB217A164B6B66A54 >Content-Type: text/x-vcard; charset=us-ascii; > name="meturner.vcf" >Content-Transfer-Encoding: 7bit >Content-Description: Card for Monte Turner >Content-Disposition: attachment; filename="meturner.vcf" begin:vcard n:Turner;Monte tel;fax:330-972-7129 tel;work:330-972-7129 x-mozilla-html:FALSE org:The University of Akron version:2.1 email;internet:meturner at uakron.edu title:Professor of Biology adr;quoted-printable:;;Dr. Monte Turner=0D=0ADepartment of Biology;Akron;Ohio;44325-3908; end:vcard --------------1D00C1CAB217A164B6B66A54-- From motani at tularik.com Wed May 12 10:38:57 1999 From: motani at tularik.com (Ali Motani) Date: Wed, 12 May 1999 09:38:57 -0600 Subject: [rat-forum] Pelleting Diet Message-ID: Does anyone know of manufacturers that supply machines for making pelleted diets from powder? From nobody at no-reply.mcw.edu Tue May 25 22:13:08 1999 From: nobody at no-reply.mcw.edu (smishra) Date: Tue, 25 May 1999 21:13:08 -0600 Subject: [rat-forum] Request Message-ID: Dear Researchers, Please I want to know the Normal concentration ranges of following in rat's blood and rat liver tissue. A. Glutathione (reduced) B. Glutathione Peroxidase C. Glutathione Reductase D. Superoxide dismutase E. catalase F. beta-carotene G. Retinol, H. L-ascorbic acid, I. Alpha Tocopherol J. Zinc, copper, Manganese, selenium and Iron Please if you do not have all the information then please tell me some other source. I need above information for academic purpose only. Thanking You sanjeev mishra sanjeevm at iitk.ac.in From gilliii at massmed.org Sat May 15 16:27:15 1999 From: gilliii at massmed.org (Thomas J. Gill) Date: Sat, 15 May 1999 15:27:15 -0600 Subject: [rat-forum] Re: rat DNA Message-ID: Hi Monte, I don't know specifically about rat DNA other that Rattus norvegicus, but I have three suggestions about contacts; Carl Hansen at NIH (NCRR) Tatsuji Nomura at the Central Insitute for Experimental Animals, Japan The contact person (don't know new one) at the Institute for Laboratory Animal Resources (ILAR) National Research Council. The Director is Ralph Dell [Tel: (202)334-2590], and he could tell you where to go. I would start here. Tom At 11:52 AM 5/12/99 0500, you wrote: >This is a multi-part message in MIME format. >--------------1D00C1CAB217A164B6B66A54 >Content-Type: text/plain; charset=us-ascii >Content-Transfer-Encoding: 7bit > > >Does anyone know of any source for either animals or DNA from a rat >species other than R. norvegicus ? > >email me for more information or any other questions. > >Thanks >Monte Turner > >--------------1D00C1CAB217A164B6B66A54 >Content-Type: text/x-vcard; charset=us-ascii; > name="meturner.vcf" >Content-Transfer-Encoding: 7bit >Content-Description: Card for Monte Turner >Content-Disposition: attachment; > filename="meturner.vcf" > >begin:vcard >n:Turner;Monte >tel;fax:330-972-7129 >tel;work:330-972-7129 >x-mozilla-html:FALSE >org:The University of Akron >version:2.1 >email;internet:meturner at uakron.edu >title:Professor of Biology >adr;quoted-printable:;;Dr. Monte Turner=0D=0ADepartment of Biology;Akron;Ohio;44325-3908; >end:vcard > >--------------1D00C1CAB217A164B6B66A54-- > Thomas J. Gill III, M.D. 64 Pond Road Duxbury, MA 02332 Tel: (781) 934-7348 Fax: (781) 934-7130 email: gilliii at massmed.org From lbrunaud at planete.net Sat May 15 23:53:47 1999 From: lbrunaud at planete.net (L. Brunaud) Date: Sat, 15 May 1999 22:53:47 -0600 Subject: [rat-forum] Re: Pelleting Diet Message-ID: >Does anyone know of manufacturers that supply machines for making pelleted >diets from powder? I had the same problem with my rats. You can mix 2 volumes powder with 1 volume of water. After that we have done pelleted diets by hands. You can cook the diet (1 hour - 50 =B0 celsius) or dry it. It works.... Dr Laurent Brunaud Digestive and endocrine surgery dpt Experimental surgery dpt CHU Brabois - Nancy =46RANCE Clementine, Benoit, Claire et Laurent BRUNAUD RICHARMENIL - FRANCE From lbrunaud at planete.net Sat May 15 23:46:19 1999 From: lbrunaud at planete.net (L. Brunaud) Date: Sat, 15 May 1999 22:46:19 -0600 Subject: [rat-forum] Precious blood ... Message-ID: I 'd like to take a sample of 1mL blood in F334 male rats (about 300g for the moment) every month during one year. Do you know a safe procedure (I don't want to kill them ) to obtain this precious blood ? Dr Laurent Brunaud Digestive and endocrine surgery dpt Experimental surgery dpt CHU Brabois - Nancy FRANCE From clkauf01 at gwise.louisville.edu Mon May 17 09:47:51 1999 From: clkauf01 at gwise.louisville.edu (Christina L. Kaufman) Date: Mon, 17 May 1999 08:47:51 -0600 Subject: [rat-forum] Re: Precious blood ... Message-ID: Hi, we routinely bleed animals for chimerism typing. The animals are warmed until a lamp (not too much!) to cause vasodilation, and then the animal is put in a restrainer, and one of the side tail veins is nicked with a sterile scalpel blade. We can easily collect a ml of blood into a ependorf tube containing 50 ul of heparin. This can be done once a week if need be. Start at the bottom of the tail, and work your way up. If the scalpel is very sharp the animal does not mind too much, and a little pressure (or even just the exposure to air) causes the would to coagulate very quickly, and the animal can be returned immeadiately to the cage. We also do this with the animals anethesized with metaphane. Christina Kaufman Christina L. Kaufman Ph.D. Institute for Cellular Therapeutics University of Louisville 500 S. Ridgeway Avenue Glenolden, PA 19306 clkauf01 at gwise.louisville.edu >>> brunaud 05/16 11:16 PM >>> I 'd like to take a sample of 1mL blood in F334 male rats (about 300g for the moment) every month during one year. Do you know a safe procedure (I don't want to kill them ) to obtain this precious blood ? Dr Laurent Brunaud Digestive and endocrine surgery dpt Experimental surgery dpt CHU Brabois - Nancy FRANCE From nobody at no-reply.mcw.edu Mon May 17 15:45:33 1999 From: nobody at no-reply.mcw.edu (mfesting) Date: Mon, 17 May 1999 14:45:33 -0600 Subject: [rat-forum] Re: rat DNA Message-ID: Another person to try would be Dr. Kazuo Moriwaki (moriwaki at soken.ac.jp). Some time ago they did a lot of work on Rattus species at the Nat. Inst. of Genetics in Mishima, Japan and they may have DNA samples there. Moriwaki no longer works there, but he would know about it. Michael > Date: Mon, 17 May 1999 08:07:29 0500 > Reply-to: rat-list at informatics.jax.org > From: "Thomas J. Gill III, M.D." > To: Multiple recipients of list > Subject: Re: rat DNA > Hi Monte, > > I don't know specifically about rat DNA other that Rattus norvegicus, but I > have three suggestions about contacts; > > Carl Hansen at NIH (NCRR) > > Tatsuji Nomura at the Central Insitute for Experimental Animals, Japan > > The contact person (don't know new one) at the Institute for Laboratory > Animal Resources (ILAR) National Research Council. The Director is > Ralph Dell [Tel: (202)334-2590], and he could tell you where to go. I > would start here. > > Tom > > > > At 11:52 AM 5/12/99 0500, you wrote: > >This is a multi-part message in MIME format. > >--------------1D00C1CAB217A164B6B66A54 > >Content-Type: text/plain; charset=us-ascii > >Content-Transfer-Encoding: 7bit > > > > > >Does anyone know of any source for either animals or DNA from a rat > >species other than R. norvegicus ? > > > >email me for more information or any other questions. > > > >Thanks > >Monte Turner > > > >--------------1D00C1CAB217A164B6B66A54 > >Content-Type: text/x-vcard; charset=us-ascii; > > name="meturner.vcf" > >Content-Transfer-Encoding: 7bit > >Content-Description: Card for Monte Turner > >Content-Disposition: attachment; > > filename="meturner.vcf" > > > >begin:vcard > >n:Turner;Monte > >tel;fax:330-972-7129 > >tel;work:330-972-7129 > >x-mozilla-html:FALSE > >org:The University of Akron > >version:2.1 > >email;internet:meturner at uakron.edu > >title:Professor of Biology > >adr;quoted-printable:;;Dr. Monte Turner=0D=0ADepartment of > Biology;Akron;Ohio;44325-3908; > >end:vcard > > > >--------------1D00C1CAB217A164B6B66A54-- > > > > > > Thomas J. Gill III, M.D. > 64 Pond Road > Duxbury, MA 02332 > Tel: (781) 934-7348 > Fax: (781) 934-7130 > email: gilliii at massmed.org > MRC Toxicology Unit, PO Box 138, Lancaster Rd., Leicester LE1 9HN FAX 44 (0) 116 252 5616 From mgarrick at acsu.buffalo.edu Mon May 17 11:21:43 1999 From: mgarrick at acsu.buffalo.edu (Michael Garrick) Date: Mon, 17 May 1999 10:21:43 -0600 Subject: [rat-forum] Re: Precious blood ... Message-ID: We routinely use light metafane anesthesia and orbital sinus puncture. So long as we alternate eyes and accept a modest anemia , we can actually bleed 1 ml every other day indefinitely for long periods providing the iron status is ok. Michael Garrick brunaud wrote: > I 'd like to take a sample of 1mL blood in F334 male rats (about 300g for > the moment) every month during one year. Do you know a safe procedure (I > don't want to kill them ) to obtain this precious blood ? > > Dr Laurent Brunaud > Digestive and endocrine surgery dpt > Experimental surgery dpt > CHU Brabois - Nancy > FRANCE -- phone 716 829-3926 before 5 PM EST 716 688-0009 after 5:30 fax 716 829-2725 mail Dr. Michael Garrick Professor of Biochemistry SUNY 140 Farber Hall Buffalo, NY 14214-3000 or 48 Bywater Drive Getzville, NY 14068-1375 email mgarrick at buffalo.edu From ken at dcm.wustl.edu Tue May 18 11:54:42 1999 From: ken at dcm.wustl.edu (Ken Boschert) Date: Tue, 18 May 1999 10:54:42 -0600 Subject: [rat-forum] Re: Precious blood ... Message-ID: A good alternative to retro-orbital bleeding, particularly for serial samples, is described and demonstrated at the following web page: http://www.uib.no/vivariet/mou_blood/Blood_coll_mice_.html I'm sure the technique can be adapted to rats. Overall, it is better for the animal and the animal handler. Ken > We routinely use light metafane anesthesia and orbital sinus puncture. So > long as we alternate eyes and accept a modest anemia , we can actually > bleed 1 ml every other day indefinitely for long periods providing the > iron status is ok. > > Michael Garrick > brunaud wrote: > > > I 'd like to take a sample of 1mL blood in F334 male rats (about 300g > > for the moment) every month during one year. Do you know a safe > > procedure (I don't want to kill them ) to obtain this precious blood ? > > > > Dr Laurent Brunaud > > Digestive and endocrine surgery dpt > > Experimental surgery dpt > > CHU Brabois - Nancy > > FRANCE > > > > -- > phone 716 829-3926 before 5 PM EST > 716 688-0009 after 5:30 > fax 716 829-2725 > mail Dr. Michael Garrick > Professor of Biochemistry > SUNY > 140 Farber Hall > Buffalo, NY 14214-3000 > or > 48 Bywater Drive > Getzville, NY 14068-1375 > email mgarrick at buffalo.edu -- Ken Boschert, DVM * Washington University * From geoff.butcher at bbsrc.ac.uk Tue May 18 17:51:05 1999 From: geoff.butcher at bbsrc.ac.uk (Geoffrey Butcher) Date: Tue, 18 May 1999 16:51:05 -0600 Subject: [rat-forum] Re: Precious blood ... Message-ID: I agree with the suggestion concerning bleeding from tail veins. There is an excellent modification, which I think came from the Dorsch and Roser laboratory. First, you keep the rat anaesthetised and warm; second you place a light weight (eg a metal rod) across the base of the tail to prevent venous return; thirdly, you use the broken-off shaft of a 21 or 22 gauge needle, slip that into the tail vein: it is then very easy and convenient to collect the blood from the other end of the shaft into an Eppendorf tube. Alternatively there is now strong support for the use of saphenous vein bleeding (see Hem et al. Laboratory Animals 32: 364-368 [1998]). This is particularly promoted as a replacement for retroorbital bleeding in mice, which is not now favoured in some establishments. Geoff Butcher From mlkal at mcw.edu Mon May 17 13:31:03 1999 From: mlkal at mcw.edu (Mary L. Kaldunski) Date: Mon, 17 May 1999 12:31:03 -0600 Subject: [rat-forum] Re: Pelleting Diet Message-ID: The last time we needed our own mixture pelleted, we shipped the powder to Dyets Inc in Pennsylvania and they mixed, pelleted, dried and shipped back. It didn't cost that much (the shipping cost the most). It was a pilot study that didn't work out so we didn't need to continue. The technical representatives at both Dyets, Inc and at Teklad Diets should be able to suggest pellet machine manufacturers and retailers to contact. Mary Kaldunski SCOR Program Coordinator Medical College of Wisconsin Milwaukee, WI 53226 414-456-8572 mlkal at mcw.edu Ali Motani wrote: > Does anyone know of manufacturers that supply machines for making pelleted > diets from powder? From nobody at no-reply.mcw.edu Wed May 19 22:13:40 1999 From: nobody at no-reply.mcw.edu (deansmart@hotmail.com) Date: Wed, 19 May 1999 21:13:40 -0600 Subject: [rat-forum] Leopoldamys Message-ID: I need taxonomic information for the genus Leopoldamys in SE Asia to check a probable new species from Thailand. Information concerning L.neilli and L.sabanus is most useful especially concerning the variability of sabanus. Also, does anybody know where I can get a chromosome karyotype carried out on the new rat at a reasonable price? What are the protocols for collecting a karyotype sample? Finally, I have collected several specimens of ectoparasite from the rats (mostly ticks, some lice). Can anyone do the identifying for me? Cheers, Dean. ********************************* Dean Smart, Cave Management Consultant, Natural Resources Conservation Office, Royal Forest Department, Phahonyothin Road, Bangkok 10900, Thailand. ______________________________________________________ Get Your Private, Free Email at http://www.hotmail.com